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ATG10 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809923

MBS809923 is an antibody targeting ATG10. Also known as ATG10 Antibody: ATTO 390. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ATG10, Polyclonal Antibody
Full Product Name
ATG10 Antibody: ATTO 390
Product Gene Name
Product Synonym Names
Rabbit Anti-Human ATG10 Polyclonal; APG 10L Antibody; APG10 like Antibody; Apg 10 Antibody; DKFZP586I0418 Antibody; ATG10 Antibody; Ubiquitin-like-conjugating enzyme ATG10 Antibody; APG10; S. cerevisiae; homolog of Antibody; ATG10_HUMAN Antibody; ATG 10 Antibody; APG10-like Antibody; Autophagy related protein 10 Antibody; APG10 Antibody; Autophagy-related protein 10 Antibody; autophagy 10; S. cerevisiae; homolog of Antibody; APG10L Antibody; ATG10 autophagy related 10 homolog Antibody; ATG10 autophagy related 10 homolog (S. cerevisiae) Antibody; FLJ13954 Antibody; PP12616 Antibody; APG10 autophagy 10 like Antibody; pp12616 Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~25 kDa.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
14,217 Da
OMIM
610800
3D Structure
ModBase 3D Structure for Q9H0Y0
Target
ATG10
Immunogen
Synthetic peptide from the mid-protein of Human ATG10
Immunogen Species
Human
Cellular Localization
Cytoplasm
Conjugate
ATTO 390
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Applications Tested/Suitable for anti-ATG10 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ATG10 antibody
WB: 1:1000
ICC/IF: 1:100

Immunocytochemistry (ICC) of anti-ATG10 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG10 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG10 Antibody . (D) Composite.
Rabbit Anti-ATG10 Polyclonal Antibody – Cat# MBS809923

Western Blot (WB) of anti-ATG10 antibody

Western blot analysis of Human HEK293 cell lysates showing detection of ~25.3 kDa ATG10 protein using Rabbit Anti-ATG10 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Hek293T cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~25.3 kDa.
Rabbit Anti-ATG10 Polyclonal Antibody, Western Blot (WB) – Cat# MBS809923

Other Notes

Small volumes of anti-ATG10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG10. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-like-conjugating enzyme ATG10
NCBI Official Synonym Full Names
autophagy related 10
NCBI Official Symbol
ATG10
NCBI Official Synonym Symbols
APG10; APG10L; pp12616
UniProt Protein Name
Ubiquitin-like-conjugating enzyme ATG10
UniProt Synonym Protein Names
Autophagy-related protein 10; APG10-like
UniProt Gene Name
ATG10
UniProt Synonym Gene Names
APG10L; APG10-like
NCBI Protein Information
ubiquitin-like-conjugating enzyme ATG10

NCBI Summary for ATG10

Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
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UniProt Comments for ATG10

E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (). Plays a role in adenovirus-mediated cell lysis.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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