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NDUFA4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821132

MBS821132 is an antibody targeting NDUFA4. Reactive in Human. Host: Rabbit. Rabbit polyclonal antibody to NDUFA4 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NDUFA4, Polyclonal Antibody
Full Product Name
Anti-NDUFA4 Antibody
Product Gene Name
anti-NDUFA4 antibody
Product Synonym Names
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4; Complex I-MLRQ; CI-MLRQ; NADH-ubiquinone oxidoreductase MLRQ subunit
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of NDUFA4 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
NDUFA4 peptide (MBS824316) is used for blocking the activity of NDUFA4 antibody (MBS821132)
Molecular Weight
9,370 Da
OMIM
phenotype 603833
3D Structure
ModBase 3D Structure for O00483
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NDUFA4. The exact sequence is proprietary.
Applications Tested/Suitable for anti-NDUFA4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-NDUFA4 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-NDUFA4 antibody

Western blot analysis of NDUFA4 expression in HeLa (A) whole cell lysates.
Rabbit Anti-NDUFA4 Polyclonal Antibody – Cat# MBS821132

Immunohistochemistry (IHC) of anti-NDUFA4 antibody

Immunohistochemical analysis of NDUFA4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-NDUFA4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS821132

Immunofluorescence (IF) of anti-NDUFA4 antibody

Immunofluorescent analysis of NDUFA4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-NDUFA4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS821132

Other Notes

Small volumes of anti-NDUFA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NDUFA4. It may not necessarily be applicable to this product.

NCBI GI #
4505357
NCBI GeneID
4697
NCBI Accession #
NP_002480.1
UniProt Primary Accession #
O00483
UniProt Secondary Accession #
Q6FHN5; A4D109
UniProt Related Accession #
O00483
GenBank Nucleotide #
NM_002489.3
NCBI Official Full Name
cytochrome c oxidase subunit NDUFA4
NCBI Official Synonym Full Names
NDUFA4, mitochondrial complex associated
NCBI Official Symbol
NDUFA4
NCBI Official Synonym Symbols
MLRQ; CI-9k; CI-MLRQ
UniProt Protein Name
Cytochrome c oxidase subunit NDUFA4
UniProt Synonym Protein Names
Complex I-MLRQ; CI-MLRQ; NADH-ubiquinone oxidoreductase MLRQ subunit
Protein Family
Cytochrome c oxidase
UniProt Gene Name
NDUFA4
UniProt Synonym Gene Names
CI-MLRQ
UniProt Entry Name
NDUA4_HUMAN
NCBI Protein Information
cytochrome c oxidase subunit NDUFA4; complex I-MLRQ; Complex I 9kDa subunit; NADH-ubiquinone oxidoreductase MLRQ subunit; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4

NCBI Summary for NDUFA4

The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq, Jul 2008]
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UniProt Comments for NDUFA4

NDUFA4: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA4 subunit family.

Protein type: Mitochondrial; Energy Metabolism - oxidative phosphorylation; Oxidoreductase; EC 1.6.5.3; EC 1.6.99.3

Chromosomal Location of Human Ortholog: 7p21.3

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex IV; mitochondrial respiratory chain complex I

Molecular Function: protein binding; cytochrome-c oxidase activity; NADH dehydrogenase (ubiquinone) activity; protein complex binding

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone
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Research Articles on NDUFA4

1. Findings support reassignment of the NDUFA4 protein to complex IV and suggest that patients with unexplained COX deficiency should be screened for NDUFA4 mutations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NDUFA4 antibody

 Reference Product  PubMed Publications
 NDUFA1 antibody  >2 publications with NDUFA4 and NDUFA1

Pathways associated with anti-NDUFA4 antibody

 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Electron Transport Chain Pathway antibodies  Electron Transport Chain Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 NADH Dehydrogenase (ubiquinone) 1 Alpha Subcomplex Pathway antibodies  NADH Dehydrogenase (ubiquinone) 1 Alpha Subcomplex Pathway Diagram
 NADH Dehydrogenase (ubiquinone) 1 Alpha Subcomplex Pathway antibodies  NADH Dehydrogenase (ubiquinone) 1 Alpha Subcomplex Pathway Diagram
 Non-alcoholic Fatty Liver Disease (NAFLD) Pathway antibodies  Non-alcoholic Fatty Liver Disease (NAFLD) Pathway Diagram

Diseases associated with anti-NDUFA4 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >6 publications with NDUFA4 and Nervous System Diseases
 Death Antibodies  >2 publications with NDUFA4 and Death
 Cognition Disorders Antibodies  >1 publications with NDUFA4 and Cognition Disorders
 Mitochondrial Diseases Antibodies  >1 publications with NDUFA4 and Mitochondrial Diseases

Organs/Tissues associated with anti-NDUFA4 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >1 publications with NDUFA4 and Kidney
 Muscle Antibodies  >1 publications with NDUFA4 and Muscle
 Liver Antibodies  >1 publications with NDUFA4 and Liver
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