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RAPGEF3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821480

MBS821480 is an antibody targeting RAPGEF3. Also known as Anti-EPAC1 Antibody. Reactive in Human, Mouse, Rat, Bovine, Dog. Host: Rabbit. Rabbit polyclonal antibody to EPAC1 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
EPAC1 (RAPGEF3), Polyclonal Antibody
Full Product Name
Anti-EPAC1 Antibody
Product Gene Name
anti-RAPGEF3 antibody
Product Synonym Names
CGEF1; EPAC; EPAC1; Rap guanine nucleotide exchange factor 3; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP; cAMP-regulated guanine nucleotide exchange factor I; cAMP-GEFI
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Dog
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of EPAC1 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
EPAC1 peptide (MBS823431) is used for blocking the activity of EPAC1 antibody (MBS821480)
Molecular Weight
99,452 Da
OMIM
606057
3D Structure
ModBase 3D Structure for O95398
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EPAC1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-RAPGEF3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)
Application Notes for anti-RAPGEF3 antibody
WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-RAPGEF3 antibody

Western blot analysis of EPAC1 expression in A549 (A), SP2/0 (B), H9C2 (C) whole cell lysates.
Rabbit Anti-RAPGEF3 Polyclonal Antibody – Cat# MBS821480

Immunofluorescence (IF) of anti-RAPGEF3 antibody

Immunofluorescent analysis of EPAC1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-RAPGEF3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS821480

Other Notes

Small volumes of anti-RAPGEF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RAPGEF3. It may not necessarily be applicable to this product.

NCBI GI #
148747862
NCBI GeneID
10411
NCBI Accession #
NP_001092002.1
UniProt Primary Accession #
O95398
UniProt Secondary Accession #
O95634; Q8WVN0; A8K2G5; E7EQC8
UniProt Related Accession #
O95398
GenBank Nucleotide #
NM_001098532.2
NCBI Official Full Name
rap guanine nucleotide exchange factor 3 isoform b
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor (GEF) 3
NCBI Official Symbol
RAPGEF3
NCBI Official Synonym Symbols
EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
UniProt Protein Name
Rap guanine nucleotide exchange factor 3
UniProt Synonym Protein Names
Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP; cAMP-regulated guanine nucleotide exchange factor I; cAMP-GEFI
Protein Family
Rap guanine nucleotide exchange factor
UniProt Gene Name
RAPGEF3
UniProt Synonym Gene Names
CGEF1; EPAC; EPAC1; EPAC 1; cAMP-GEFI
UniProt Entry Name
RPGF3_HUMAN
NCBI Protein Information
rap guanine nucleotide exchange factor 3; EPAC 1; 9330170P05Rik; exchange factor directly activated by cAMP 1; exchange protein directly activated by cAMP 1; cAMP-regulated guanine nucleotide exchange factor I; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP

UniProt Comments for RAPGEF3

Epac1: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, misc.; GEFs

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: membrane; plasma membrane; endomembrane system; cAMP-dependent protein kinase complex

Molecular Function: protein binding; Rap guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; platelet activation; signal transduction; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein export from nucleus; cell proliferation; positive regulation of angiogenesis; cAMP-mediated signaling; positive regulation of stress fiber formation; energy reserve metabolic process; positive regulation of cAMP catabolic process; angiogenesis; regulation of protein kinase activity; blood coagulation; regulation of insulin secretion; Rap protein signal transduction
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Research Articles on RAPGEF3

1. cAMP binding to EPAC1 may involve, in addition to the waters from within the first coordination sphere, also some waters from the second coordination sphere of the protein and cAMP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RAPGEF3 antibody

 Reference Product  PubMed Publications
 SOCS3 antibody  >8 publications with RAPGEF3 and SOCS3
 RAP1A antibody  >7 publications with RAPGEF3 and RAP1A
 RAP1B antibody  >6 publications with RAPGEF3 and RAP1B
 RANBP2 antibody  >2 publications with RAPGEF3 and RANBP2
 RRAS antibody  >1 publications with RAPGEF3 and RRAS

Pathways associated with anti-RAPGEF3 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway antibodies  Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Integration Of Energy Metabolism Pathway antibodies  Integration Of Energy Metabolism Pathway Diagram
 Integrin AlphaIIb Beta3 Signaling Pathway antibodies  Integrin AlphaIIb Beta3 Signaling Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram

Diseases associated with anti-RAPGEF3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >15 publications with RAPGEF3 and Inflammation
 Hypertrophy Antibodies  >12 publications with RAPGEF3 and Hypertrophy
 Edema Antibodies  >5 publications with RAPGEF3 and Edema
 Necrosis Antibodies  >5 publications with RAPGEF3 and Necrosis
 Neoplasms, Experimental Antibodies  >4 publications with RAPGEF3 and Neoplasms, Experimental
 Pancreatic Neoplasms Antibodies  >3 publications with RAPGEF3 and Pancreatic Neoplasms
 Kidney Diseases Antibodies  >2 publications with RAPGEF3 and Kidney Diseases
 Pulmonary Disease, Chronic Obstructive Antibodies  >2 publications with RAPGEF3 and Pulmonary Disease, Chronic Obstructive
 Lung Neoplasms Antibodies  >1 publications with RAPGEF3 and Lung Neoplasms
 Uterine Cervical Neoplasms Antibodies  >1 publications with RAPGEF3 and Uterine Cervical Neoplasms

Organs/Tissues associated with anti-RAPGEF3 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >40 publications with RAPGEF3 and Vascular
 Blood Antibodies  >35 publications with RAPGEF3 and Blood
 Heart Antibodies  >27 publications with RAPGEF3 and Heart
 Muscle Antibodies  >27 publications with RAPGEF3 and Muscle
 Lung Antibodies  >20 publications with RAPGEF3 and Lung
 Brain Antibodies  >15 publications with RAPGEF3 and Brain
 Pancreas Antibodies  >13 publications with RAPGEF3 and Pancreas
 Kidney Antibodies  >9 publications with RAPGEF3 and Kidney
 Umbilical Cord Antibodies  >6 publications with RAPGEF3 and Umbilical Cord
 Placenta Antibodies  >5 publications with RAPGEF3 and Placenta
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