IGFBP2 ELISA Kit for Mouse

Background: Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

Principle of the Assay: The MyBioSource' Mouse IGFBP2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP2 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse IGFBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Small volumes of IGFBP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS824790 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IGFBP2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGFBP2. The ELISA analytical biochemical technique of the MBS824790 kit is based on IGFBP2 antibody-IGFBP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).