A Disintegrin Like and Metalloproteinase with Thrombospondin Type 1 Motif 4 (ADAMTS4) ELISA Kit for Rat

Name: Rat ADAMTS4 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS008272
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS008272

Background: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

A Disintegrin Like and Metalloproteinase With Thrombospondin Type 1 Motif 4 (ADAMTS4), ELISA Kit

Full Product Name

Rat A Disintegrin Like and Metalloproteinase With Thrombospondin Type 1 Motif 4 ELISA Kit

Product Gene Name

ADAMTS4 elisa kit

Species Reactivity

Rat

Samples

Serum, Plasma, Tissue Homogenate, Urine and other Body Fluids

Detection Range

6.25 - 200 ng/ml.

Preparation and Storage

Store all reagents at 2-8 degree C

Molecular Weight

90,197 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (161159538..161168845, complement). Location: 1q21-q23

OMIM

603876

3D Structure

ModBase 3D Structure for O75173

Intended Uses

This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Rat ADAMTS4 in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Rat ADAMTS4 concentration. The concentration of Rat ADAMTS4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of ADAMTS4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADAMTS4 purchase

MBS008272 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin Like and Metalloproteinase With Thrombospondin Type 1 Motif 4 (ADAMTS4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTS4. The ELISA analytical biochemical technique of the MBS008272 kit is based on ADAMTS4 antibody-ADAMTS4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ADAMTS4. It may not necessarily be applicable to this product.

NCBI GI #

157427675

NCBI GeneID

9507

NCBI Accession #

NP_005090.3

UniProt Primary Accession #

O75173

UniProt Secondary Accession #

Q5VTW2; Q6P4Q8; Q6UWA8; Q9UN83

UniProt Related Accession #

O75173

GenBank Nucleotide #

NM_005099.4

NCBI Official Full Name

A disintegrin and metalloproteinase with thrombospondin motifs 4 preproprotein

NCBI Official Synonym Full Names

ADAM metallopeptidase with thrombospondin type 1 motif, 4

NCBI Official Symbol

ADAMTS4

NCBI Official Synonym Symbols

ADMP-1; ADAMTS-2; ADAMTS-4

UniProt Protein Name

A disintegrin and metalloproteinase with thrombospondin motifs 4

UniProt Synonym Protein Names

ADMP-1; Aggrecanase-1

Protein Family

A disintegrin and metalloproteinase with thrombospondin motifs

UniProt Gene Name

ADAMTS4

UniProt Synonym Gene Names

KIAA0688; ADAM-TS 4; ADAM-TS4; ADAMTS-4

UniProt Entry Name

ATS4_HUMAN

NCBI Protein Information

A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4

NCBI Summary for ADAMTS4

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq, Jul 2008]

UniProt Comments for ADAMTS4

ADAMTS4: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

Protein type: Secreted, signal peptide; EC 3.4.24.82; Protease; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 1q21-q23

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: peptidase activity; protein binding; protease binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response to bacterium; proteolysis; skeletal development

Research Articles on ADAMTS4

1. AMTS4 has roles in melanoma growth and angiogenesis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ADAMTS4 elisa kit

Reference Product	PubMed Publications
ACAN elisa kit	>6 publications with ADAMTS4 and ACAN
BCAN elisa kit	>3 publications with ADAMTS4 and BCAN
TGFB1 elisa kit	>3 publications with ADAMTS4 and TGFB1
FURIN elisa kit	>3 publications with ADAMTS4 and FURIN
OSM elisa kit	>2 publications with ADAMTS4 and OSM
IL1B elisa kit	>2 publications with ADAMTS4 and IL1B
IL17A elisa kit	>1 publications with ADAMTS4 and IL17A
SRPX2 elisa kit	>1 publications with ADAMTS4 and SRPX2

Pathways associated with ADAMTS4 elisa kit

Products by Pathway	Pathway Diagram
Endochondral Ossification Pathway antibodies	Endochondral Ossification Pathway Diagram

Diseases associated with ADAMTS4 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>16 publications with ADAMTS4 and Inflammation
Neoplasms Antibodies	>14 publications with ADAMTS4 and Neoplasms
Necrosis Antibodies	>14 publications with ADAMTS4 and Necrosis
Arthritis, Rheumatoid Antibodies	>7 publications with ADAMTS4 and Arthritis, Rheumatoid
Nervous System Diseases Antibodies	>5 publications with ADAMTS4 and Nervous System Diseases
Atherosclerosis Antibodies	>5 publications with ADAMTS4 and Atherosclerosis
Brain Diseases Antibodies	>5 publications with ADAMTS4 and Brain Diseases
Heart Diseases Antibodies	>4 publications with ADAMTS4 and Heart Diseases
Myocardial Infarction Antibodies	>4 publications with ADAMTS4 and Myocardial Infarction
Carcinoma, Squamous Cell Antibodies	>2 publications with ADAMTS4 and Carcinoma, Squamous Cell

Organs/Tissues associated with ADAMTS4 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>13 publications with ADAMTS4 and Brain
Muscle Antibodies	>4 publications with ADAMTS4 and Muscle
Embryonic Tissue Antibodies	>3 publications with ADAMTS4 and Embryonic Tissue
Lung Antibodies	>1 publications with ADAMTS4 and Lung