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NCALD Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827514

MBS827514 is an antibody targeting NCALD. Reactive in Human, Mouse, Rat, Bovine, Chicken, Monkey. Host: Rabbit. Rabbit polyclonal antibody to NCALD More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NCALD, Polyclonal Antibody
Full Product Name
Anti-NCALD Antibody
Product Gene Name
Product Synonym Names
Neurocalcin-delta
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Monkey
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of NCALD protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
NCALD peptide (MBS8213511) is used for blocking the activity of NCALD antibody (MBS827514)
Molecular Weight
22,245 Da
OMIM
606722
3D Structure
ModBase 3D Structure for P61601
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NCALD. The exact sequence is proprietary.
Applications Tested/Suitable for anti-NCALD antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NCALD antibody
WB (1/500 - 1/1000), IHC (1/100 - 1/200)

Western Blot (WB) of anti-NCALD antibody

Western blot analysis of NCALD expression in HeLa (A), NS-1 (B), H9C2 (C) whole cell lysates.
Rabbit Anti-NCALD Polyclonal Antibody – Cat# MBS827514

Immunohistochemistry (IHC) of anti-NCALD antibody

Immunohistochemical analysis of NCALD staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-NCALD Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS827514

Other Notes

Small volumes of anti-NCALD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NCALD. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neurocalcin-delta
NCBI Official Synonym Full Names
neurocalcin delta
NCBI Official Symbol
NCALD
UniProt Protein Name
Neurocalcin-delta
Protein Family
UniProt Gene Name
NCALD
UniProt Entry Name
NCALD_HUMAN
NCBI Protein Information
neurocalcin-delta

NCBI Summary for NCALD

This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for NCALD

NCALD: May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions. Belongs to the recoverin family.

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: clathrin coat of trans-Golgi network vesicle; intracellular; cytosol

Molecular Function: tubulin binding; clathrin binding; protein binding; calcium ion binding; actin binding; alpha-tubulin binding

Biological Process: vesicle-mediated transport; synaptic transmission; regulation of systemic arterial blood pressure; calcium-mediated signaling
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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