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LYPLA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829216

MBS829216 is an antibody targeting LYPLA1. Reactive in Human, Mouse, Rat, Rabbit. Host: Rabbit. Rabbit polyclonal antibody to LYPLA1 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LYPLA1, Polyclonal Antibody
Full Product Name
Anti-LYPLA1 Antibody
Product Gene Name
anti-LYPLA1 antibody
Product Synonym Names
APT1; LPL1; Acyl-protein thioesterase 1; APT-1; hAPT1; Lysophospholipase 1; Lysophospholipase I; LPL-I; LysoPLA I
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Rabbit
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of LYPLA1 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
LYPLA1 peptide (MBS8232713) is used for blocking the activity of LYPLA1 antibody (MBS829216)
Molecular Weight
22,875 Da
OMIM
605599
3D Structure
ModBase 3D Structure for O75608
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LYPLA1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-LYPLA1 antibody
Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-LYPLA1 antibody
WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF (1/100 - 1/500), ICC (1/100 - 1/500)

Western Blot (WB) of anti-LYPLA1 antibody

Western blot analysis of LYPLA1 expression in MDAMB453 (A), mouse kidney (B), rat kidney (C) whole cell lysates.
Rabbit Anti-LYPLA1 Polyclonal Antibody – Cat# MBS829216

Immunohistochemistry (IHC) of anti-LYPLA1 antibody

Immunohistochemical analysis of LYPLA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-LYPLA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS829216

Immunofluorescence (IF) of anti-LYPLA1 antibody

Immunofluorescent analysis of LYPLA1 staining in MDAMB453 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-LYPLA1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS829216

Other Notes

Small volumes of anti-LYPLA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LYPLA1. It may not necessarily be applicable to this product.

NCBI GI #
525342559
NCBI GeneID
10434
NCBI Accession #
NP_001266285.1
UniProt Primary Accession #
O75608
UniProt Secondary Accession #
O43202; Q9UQF9
UniProt Related Accession #
O75608
GenBank Nucleotide #
NM_001279356.1
NCBI Official Full Name
acyl-protein thioesterase 1 isoform 2
NCBI Official Synonym Full Names
lysophospholipase I
NCBI Official Symbol
LYPLA1
NCBI Official Synonym Symbols
APT1; LPL1; APT-1; LPL-I; hAPT1
UniProt Protein Name
Acyl-protein thioesterase 1
UniProt Synonym Protein Names
Lysophospholipase 1; Lysophospholipase I; LPL-I; LysoPLA I
UniProt Gene Name
LYPLA1
UniProt Synonym Gene Names
APT1; LPL1; APT-1; hAPT1; LPL-I; LysoPLA I
UniProt Entry Name
LYPA1_HUMAN
NCBI Protein Information
acyl-protein thioesterase 1

NCBI Summary for LYPLA1

This gene encodes a member of the alpha/beta hydrolase superfamily. The encoded protein functions as a homodimer, exhibiting both depalmitoylating as well as lysophospholipase activity, and may be involved in Ras localization and signaling. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 4, 6, and 7. [provided by RefSeq, Jul 2013]
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UniProt Comments for LYPLA1

LYPLA1: Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity and also low lysophospholipase activity. Belongs to the AB hydrolase 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.2.-; Phospholipase; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 8q11.23

Cellular Component: mitochondrion; cytosol

Molecular Function: palmitoyl-(protein) hydrolase activity; lysophospholipase activity

Biological Process: negative regulation of Golgi to plasma membrane protein transport; regulation of nitric-oxide synthase activity; fatty acid metabolic process; nitric oxide metabolic process; protein depalmitoylation
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Research Articles on LYPLA1

1. High expression of APT1 is associated with chronic lymphocytic leukemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-LYPLA1 antibody

 Products by Pathway  Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nitric Oxide Pathway antibodies  Metabolism Of Nitric Oxide Pathway Diagram
 ENOS Activation Pathway antibodies  ENOS Activation Pathway Diagram
 ENOS Activation And Regulation Pathway antibodies  ENOS Activation And Regulation Pathway Diagram

Diseases associated with anti-LYPLA1 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >3 publications with LYPLA1 and Carcinoma
 Hyperplasia Antibodies  >2 publications with LYPLA1 and Hyperplasia
 Cardiovascular Diseases Antibodies  >1 publications with LYPLA1 and Cardiovascular Diseases
 Neoplasm Metastasis Antibodies  >1 publications with LYPLA1 and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >1 publications with LYPLA1 and Neoplasms, Experimental
 Atherosclerosis Antibodies  >1 publications with LYPLA1 and Atherosclerosis
 Urinary Bladder Neoplasms Antibodies  >1 publications with LYPLA1 and Urinary Bladder Neoplasms
 Necrosis Antibodies  >1 publications with LYPLA1 and Necrosis

Organs/Tissues associated with anti-LYPLA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with LYPLA1 and Blood
 Brain Antibodies  >6 publications with LYPLA1 and Brain
 Stomach Antibodies  >5 publications with LYPLA1 and Stomach
 Kidney Antibodies  >3 publications with LYPLA1 and Kidney
 Vascular Antibodies  >2 publications with LYPLA1 and Vascular
 Prostate Antibodies  >2 publications with LYPLA1 and Prostate
 Muscle Antibodies  >1 publications with LYPLA1 and Muscle
 Ovary Antibodies  >1 publications with LYPLA1 and Ovary
 Bladder Antibodies  >1 publications with LYPLA1 and Bladder
 Skin Antibodies  >1 publications with LYPLA1 and Skin
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