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PIN 1 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS836998

MBS836998 is an active protein targeting Pin 1. Also known as Pin 1 protein. Host: Human. Purified recombinant Human Pin 1 protein More details

$755 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Pin 1, Active Protein
Full Product Name
Pin 1 protein
Product Gene Name
Pin 1 active protein
Product Synonym Names
Rotamase Pin1 protein; UBL5 protein; PPIase protein; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein; Pin -1 protein; Pin -1; Pin 1; PPIase Pin1 protein; Pin 1 protein; DOD protein; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; PIN1 protein; EC 5.2.1.8 protein
Sequence
Extracellu lar Ig-like domain; 22-255 amino acids: MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE
Host
Human
Form/Format
Supplied as a liquid in 20mM Tris-HCl buffer, pH7.5, containing 0.1M NaCl, 5mM DTT, and 20% glycerol.
Purity/Purification
> 95% pure
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
18.2 kDa (163 aa), confirmed by MALDI-TOF.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Protein Type
Recombinant
Bioactivity
Specific activity is > 162 nM/min/ug, and is defined as the amount of enzyme that cleaves 1 uM of suc-AAFP-pNA per minute at 1 degree C in Tris-Hcl pH 8.0 using chymotrypsin.
Endotoxin Levels
< 1.0 EU per ug of protein (determined by LAL method)
Expression System
E Coli

Product Categories/Family for Pin 1 active protein

Cell Cycle & Cell Death
Applications Tested/Suitable for Pin 1 active protein
SDS-PAGE
Application Notes for Pin 1 active protein
1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Pin 1 protein at various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes.

SDS-PAGE of Pin 1 active protein

Figure annotation denotes ug of protein loaded and % gel used.
PIN 1 Active Protein – Cat# MBS836998

Other Notes

Small volumes of Pin 1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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