• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Apolipoprotein E (APOE) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9601351

Description: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain,… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Apolipoprotein E (APOE), Polyclonal Antibody
Full Product Name
Apolipoprotein E Antibody
Product Gene Name
anti-APOE antibody
Product Synonym Names
AD2; Apo-E; APOE; APOE_HUMAN; APOEA; Apolipoprotein E; Apolipoprotein E3; ApolipoproteinE; Apoprotein; LDLCQ5; LPG
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
Apolipoprotein E antibody detects endogenous levels of total Apolipoprotein E
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
APOE peptide (MBS9616883) is used for blocking the activity of APOE antibody (MBS9601351)
Molecular Weight
Observed: 36 kDa
Predicted: 37 kDa
3D Structure
ModBase 3D Structure for P02649
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Apolipoprotein E
Subcellular Location
Secreted.
Tissue Specificity
Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
Predicted Cross Reactivity
Bovine, Rabbit
Similarity
Bovine (82%), Rabbit (92%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-APOE antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-APOE antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunohistochemistry (IHC) of anti-APOE antibody

MBS9601351 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-APOE Polyclonal Antibody – Cat# MBS9601351

Immunofluorescene (IF) of anti-APOE antibody

MBS9601351 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-APOE Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9601351

Western Blot (WB) of anti-APOE antibody

Western blot analysis of Apolipoprotein E expression in RAW264.7 cells, The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-APOE Polyclonal Antibody, Western Blot (WB) – Cat# MBS9601351

Other Notes

Small volumes of anti-APOE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APOE purchase

MBS9601351 is an antibody targeting APOE. Also known as Apolipoprotein E Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for APOE. It may not necessarily be applicable to this product.

NCBI GI #
4557325
NCBI GeneID
348
NCBI Accession #
NP_000032.1
UniProt Primary Accession #
P02649
UniProt Secondary Accession #
Q9P2S4; B2RC15; C0JYY5
UniProt Related Accession #
P02649
GenBank Nucleotide #
NM_000041.3
NCBI Official Full Name
apolipoprotein E isoform b
NCBI Official Synonym Full Names
apolipoprotein E
NCBI Official Symbol
APOE
NCBI Official Synonym Symbols
AD2; LPG; APO-E; ApoE4; LDLCQ5
UniProt Protein Name
Apolipoprotein E
Protein Family
Apolipoprotein
UniProt Gene Name
APOE
UniProt Synonym Gene Names
Apo-E
NCBI Protein Information
apolipoprotein E

NCBI Summary for APOE

The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016]
Show more

UniProt Comments for APOE

Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.

Research Articles on APOE

1. Site-directed mutagenesis of Akt at Cys224 revealed that S-nitrosylation at this site was pivotal for the reduced phosphorylation at Akt Ser473, which led to impaired Akt signaling. Furthermore, on HHcy challenge, as compared with GSNOR(+/+)ApoE(-/-) littermate controls, GSNOR(-/-)ApoE(-/-) double knockout mice showed reduced T-cell activation with concurrent reduction of atherosclerosis.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-APOE antibody

 Reference Product  PubMed Publications
 LDLR antibody  >462 publications with APOE and LDLR
 APP antibody  >433 publications with APOE and APP
 SCARB1 antibody  >100 publications with APOE and SCARB1
 MAPT antibody  >82 publications with APOE and MAPT
 LRP1 antibody  >62 publications with APOE and LRP1
 A2M antibody  >62 publications with APOE and A2M
 PLTP antibody  >47 publications with APOE and PLTP
 VLDLR antibody  >44 publications with APOE and VLDLR
 PRNP antibody  >40 publications with APOE and PRNP
 LRP8 antibody  >13 publications with APOE and LRP8

Pathways associated with anti-APOE antibody

 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 HDL-mediated Lipid Transport Pathway antibodies  HDL-mediated Lipid Transport Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Diseases associated with anti-APOE antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >5315 publications with APOE and Cardiovascular Diseases
 Alzheimer Disease Antibodies  >4934 publications with APOE and Alzheimer Disease
 Atherosclerosis Antibodies  >4115 publications with APOE and Atherosclerosis
 Arteriosclerosis Antibodies  >3499 publications with APOE and Arteriosclerosis
 Hyperlipidemias Antibodies  >1711 publications with APOE and Hyperlipidemias
 Inflammation Antibodies  >1227 publications with APOE and Inflammation
 Cognition Disorders Antibodies  >1082 publications with APOE and Cognition Disorders
 Coronary Disease Antibodies  >1041 publications with APOE and Coronary Disease
 Hypercholesterolemia Antibodies  >969 publications with APOE and Hypercholesterolemia
 Plaque, Atherosclerotic Antibodies  >949 publications with APOE and Plaque, Atherosclerotic

Organs/Tissues associated with anti-APOE antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3719 publications with APOE and Brain
 Vascular Antibodies  >3484 publications with APOE and Vascular
 Liver Antibodies  >1673 publications with APOE and Liver
 Heart Antibodies  >1327 publications with APOE and Heart
 Muscle Antibodies  >1014 publications with APOE and Muscle
 Nerve Antibodies  >573 publications with APOE and Nerve
 Bone Antibodies  >481 publications with APOE and Bone
 Kidney Antibodies  >425 publications with APOE and Kidney
 Lung Antibodies  >229 publications with APOE and Lung
 Spleen Antibodies  >191 publications with APOE and Spleen
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000