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HA-TAG Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS840096

Background: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
HA-Tag, Monoclonal Antibody
Full Product Name
HA-Tag Antibody (Clone 12CA5[2])
Product Gene Name
Product Synonym Names
GRIN2A; hNR2A; NMDAR2A; NR2A
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
12CA5[2]
Host
Mouse
Species Reactivity
All species
Form/Format
200 ug (1 mg/ml) anti-HA-Tag monoclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Appearance: Colorless liquid
Purity/Purification
Affinity purified
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
62,693 Da
Immunogen
Synthetic peptide (YPYDVPDYA) derived from the human influenza hemaglutinin protein
Handling
The antibody solution should be gently mixed before use.
Applications Tested/Suitable for anti-HA-Tag antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes for anti-HA-Tag antibody
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml), and immunocytochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected mammalian cells.

Other Notes

Small volumes of anti-HA-Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HA-Tag purchase

MBS840096 is an antibody targeting HA-Tag. Also known as HA-Tag Antibody (Clone 12CA5[2]). Reactive in All species. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HA-Tag. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
hemagglutinin, partial
UniProt Protein Name
Hemagglutinin
UniProt Gene Name
HA
UniProt Entry Name
D5XIF4_9INFA

UniProt Comments for HA-Tag

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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