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ICAM1/CD54 (ICAM1) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844403

Background: Inter-Cellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54), is a member of the immunoglobulin superfamily, and is a cell surface glycoprotein which is typically expressed in low concentrations on endothelial… More details

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Product Overview

Product Name
ICAM1/CD54, Active Protein
Full Product Name
Human CellExp ICAM1 / CD54, human recombinant
Product Gene Name
ICAM1 active protein
Product Synonym Names
ICAM1; ICAM-1; BB2; BB-2; CD54; CD-54; P3.58
Host
HEK293 cells
Form/Format
Lyophilized from 0.22 mum filtered solution in 50 mM Tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized
Purity/Purification
>=98%
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
This protein is fused with Fc fragment of human IgG1 at the C-terminus and has a calculated MW of 75.7 kDa expressed. The predicted N-terminus is Gln 27. Protein migrates as 100-110 kDa in reduced SDS-PAGE resulting from glycosylation.
OMIM
147840
3D Structure
ModBase 3D Structure for P05362
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
Endotoxin Level
<1 EU/mug by LAL method
Biological Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5×104 cells/well are added to rhICAM/Fc Chimera coated plates (12.5 mug/ml with 100 mul/well), >60% will adhere after PMA 1 hour incubation at 37 degree C.
Results
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5×104 cells/well are added to rhICAM/Fc Chimera coated plates (12.5 mug/ml with 100 mul/well), >60% will adhere after PMA 1 hour incubation at 37 degree C.
Handling
Centrifuge the vial prior to opening.

Product Categories/Family for ICAM1 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; ICAMsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z
Applications Tested/Suitable for ICAM1 active protein
SDS-PAGE

Testing Data of ICAM1 active protein

ICAM1 Active Protein – Cat# MBS844403

Other Notes

Small volumes of ICAM1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ICAM1 purchase

MBS844403 is an active protein targeting ICAM1. Also known as Human CellExp ICAM1 / CD54, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for ICAM1. It may not necessarily be applicable to this product.

NCBI GI #
167466198
NCBI GeneID
3383
NCBI Accession #
NP_000192.2
UniProt Primary Accession #
P05362
UniProt Secondary Accession #
Q5NKV7; Q96B50; B2R6M3
UniProt Related Accession #
P05362
GenBank Nucleotide #
NM_000201.2
NCBI Official Full Name
intercellular adhesion molecule 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 1
NCBI Official Symbol
ICAM1
NCBI Official Synonym Symbols
BB2; CD54; P3.58
UniProt Protein Name
Intercellular adhesion molecule 1
UniProt Synonym Protein Names
Major group rhinovirus receptor; CD_antigen: CD54
Protein Family
Intercellular adhesion molecule
UniProt Gene Name
ICAM1
UniProt Synonym Gene Names
ICAM-1
UniProt Entry Name
ICAM1_HUMAN
NCBI Protein Information
intercellular adhesion molecule 1

NCBI Summary for ICAM1

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

UniProt Comments for ICAM1

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Cell adhesion; Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; cell surface; focal adhesion; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane

Molecular Function: viral receptor activity; integrin binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: extracellular matrix organization and biogenesis; entry of virus into host cell; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; cellular response to nutrient levels; leukocyte adhesion; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; regulation of leukocyte mediated cytotoxicity; acute inflammatory response to antigenic stimulus; regulation of cell adhesion; response to drug; virion attachment, binding of host cell surface receptor; negative regulation of calcium ion transport; regulation of immune response; cytokine and chemokine mediated signaling pathway; response to amphetamine; cell aging; response to amino acid stimulus; heterophilic cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; positive regulation of actin filament polymerization; response to copper ion; positive regulation of vasoconstriction; cell adhesion mediated by integrin; adhesion to host; response to ionizing radiation; leukocyte migration

Disease: Malaria, Susceptibility To
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Research Articles on ICAM1

1. Our results support that osteopontin regulates ICAM-1 and VEGFA expression mainly in triple-negative/basal-like breast carcinomas

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ICAM1 active protein

 Reference Product  PubMed Publications
 TNF active protein  >3430 publications with ICAM1 and TNF
 ITGB2 active protein  >886 publications with ICAM1 and ITGB2
 ITGAL active protein  >478 publications with ICAM1 and ITGAL
 ITGAM active protein  >445 publications with ICAM1 and ITGAM
 RELA active protein  >223 publications with ICAM1 and RELA
 ITGAX active protein  >141 publications with ICAM1 and ITGAX
 VCAM1 active protein  >109 publications with ICAM1 and VCAM1
 NFKB1 active protein  >21 publications with ICAM1 and NFKB1
 IRF1 active protein  >17 publications with ICAM1 and IRF1

Pathways associated with ICAM1 active protein

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram

Diseases associated with ICAM1 active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5812 publications with ICAM1 and Inflammation
 Necrosis Antibodies  >4893 publications with ICAM1 and Necrosis
 Cardiovascular Diseases Antibodies  >3750 publications with ICAM1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >1516 publications with ICAM1 and Atherosclerosis
 Heart Diseases Antibodies  >1043 publications with ICAM1 and Heart Diseases
 Reperfusion Injury Antibodies  >906 publications with ICAM1 and Reperfusion Injury
 Myocardial Ischemia Antibodies  >817 publications with ICAM1 and Myocardial Ischemia
 Kidney Diseases Antibodies  >778 publications with ICAM1 and Kidney Diseases
 Hypertension Antibodies  >542 publications with ICAM1 and Hypertension
 Asthma Antibodies  >507 publications with ICAM1 and Asthma

Organs/Tissues associated with ICAM1 active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11984 publications with ICAM1 and Blood
 Vascular Antibodies  >9000 publications with ICAM1 and Vascular
 Lung Antibodies  >1808 publications with ICAM1 and Lung
 Heart Antibodies  >1324 publications with ICAM1 and Heart
 Brain Antibodies  >1210 publications with ICAM1 and Brain
 Skin Antibodies  >1206 publications with ICAM1 and Skin
 Liver Antibodies  >1202 publications with ICAM1 and Liver
 Kidney Antibodies  >1199 publications with ICAM1 and Kidney
 Muscle Antibodies  >1069 publications with ICAM1 and Muscle
 Embryonic Tissue Antibodies  >785 publications with ICAM1 and Embryonic Tissue
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