Human Cellexp TFPI Active Protein – Cat# MBS844659

TFPI Human Cellexp Active Protein

Name: TFPI Active Protein; Human Cellexp; MyBioSource
Brand: MyBioSource
SKU: MBS844659
Price: 350 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844659

Background: Tissue factor pathway inhibitor (TFPI) also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TFPI, Active Protein

Full Product Name

Human CellExp TFPI, human recombinant

Product Gene Name

TFPI active protein

Product Synonym Names

TFPI; LACI; TFPI1; EPI; TFI

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 30 kDa. The predicted N-terminus is Asp 29. DTT-reduced Protein migrates as 41-45 kDa due to glycosylation.

OMIM

152310

3D Structure

ModBase 3D Structure for P10646

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK (Dnp) - NH2. The IC50 value is < 0.35 nM, as measured in 100 mul reaction mixture containing 1.25 ng trypsin, 10 muM substrate, 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5.

Results

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for TFPI active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes

Applications Tested/Suitable for TFPI active protein

SDS-PAGE

Other Notes

Small volumes of TFPI active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFPI purchase

MBS844659 is an active protein targeting TFPI. Also known as Human CellExp TFPI, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for TFPI. It may not necessarily be applicable to this product.

NCBI GI #

73760409

NCBI GeneID

7035

NCBI Accession #

NP_001027452.1

UniProt Primary Accession #

P10646

UniProt Secondary Accession #

O95103; Q53TS4

UniProt Related Accession #

P10646

GenBank Nucleotide #

NM_001032281.2

NCBI Official Full Name

tissue factor pathway inhibitor isoform b

NCBI Official Synonym Full Names

tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)

NCBI Official Symbol

TFPI

NCBI Official Synonym Symbols

EPI; TFI; LACI; TFPI1

UniProt Protein Name

Tissue factor pathway inhibitor

UniProt Synonym Protein Names

Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI

Protein Family

Tissue factor pathway inhibitor

UniProt Gene Name

TFPI

UniProt Synonym Gene Names

LACI; TFPI1; TFPI; EPI; LACI

UniProt Entry Name

TFPI1_HUMAN

NCBI Protein Information

tissue factor pathway inhibitor

NCBI Summary for TFPI

This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]

UniProt Comments for TFPI

TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: extracellular space; cell surface; endoplasmic reticulum; extracellular region; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: blood coagulation, extrinsic pathway; blood coagulation

Research Articles on TFPI

1. Colon TFPI levels are increased in ulcerative colitis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TFPI active protein

Reference Product	PubMed Publications
F10 active protein	>5 publications with TFPI and F10
SON active protein	>2 publications with TFPI and SON
SDC4 active protein	>1 publications with TFPI and SDC4

Pathways associated with TFPI active protein

Products by Pathway	Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Extrinsic Pathway Of Fibrin Clot Formation antibodies	Extrinsic Pathway Of Fibrin Clot Formation Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Integrated Breast Cancer Pathway antibodies	Integrated Breast Cancer Pathway Diagram
Syndecan-4-mediated Signaling Events Pathway antibodies	Syndecan-4-mediated Signaling Events Pathway Diagram

Diseases associated with TFPI active protein

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>540 publications with TFPI and Cardiovascular Diseases
Inflammation Antibodies	>203 publications with TFPI and Inflammation
Heart Diseases Antibodies	>134 publications with TFPI and Heart Diseases
Venous Thrombosis Antibodies	>131 publications with TFPI and Venous Thrombosis
Necrosis Antibodies	>79 publications with TFPI and Necrosis
Myocardial Infarction Antibodies	>66 publications with TFPI and Myocardial Infarction
Kidney Diseases Antibodies	>58 publications with TFPI and Kidney Diseases
Neoplasm Metastasis Antibodies	>39 publications with TFPI and Neoplasm Metastasis
Pain Antibodies	>38 publications with TFPI and Pain
Adenocarcinoma Antibodies	>36 publications with TFPI and Adenocarcinoma

Organs/Tissues associated with TFPI active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1283 publications with TFPI and Blood
Vascular Antibodies	>547 publications with TFPI and Vascular
Embryonic Tissue Antibodies	>105 publications with TFPI and Embryonic Tissue
Muscle Antibodies	>100 publications with TFPI and Muscle
Lung Antibodies	>95 publications with TFPI and Lung
Heart Antibodies	>91 publications with TFPI and Heart
Liver Antibodies	>81 publications with TFPI and Liver
Kidney Antibodies	>80 publications with TFPI and Kidney
Placenta Antibodies	>48 publications with TFPI and Placenta
Umbilical Cord Antibodies	>47 publications with TFPI and Umbilical Cord