Cd-14 (CD14) Active Protein

Name: CD14 Active Protein; Cd-14; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844753
Price: 555 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844753

Background: CD14 acts as a receptor for endotoxin (LPS), is expressed strongly on monocy¬tes, macrophages and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CD-14 (CD14), Active Protein

Full Product Name

CD-14, mouse recombinant

Product Gene Name

CD14 active protein

Product Synonym Names

CD14; Cluster of differentiation 14; Human CD14; CD14; h-CD14; rh-CD14; recombinant human CD14; recombinant CD14; CD14; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14; Cluster of differentiation 14; mouse CD14; CD14; h-CD14; rm-CD14; recombinant mouse CD14; recombinant CD14; CD14

Host

CHO Cells

Form/Format

Lyophilized from a concentrated protein solution (1 mg/ml) containing PBS, pH 7.2
Appearance: Lyophilized protein

Purity/Purification

90-95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

50.0 kDa

3D Structure

ModBase 3D Structure for P10810

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Reconstitute in 10 ul of ultra-pure distilled water. This solution can then be diluted into other aqueous buffers and stored at -20 degree C or -70 degree C for future use.

Endotoxin Level

<0.1 ng/mug

Biological Activity

Inhibits binding of LPS to CD14. Up to 5ug/ml mouse CD14 inhibit binding of FITC-LPS (0.5ug/ml) to mouse CD14+ CHO transfectants

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for CD14 active protein

Cytokines; Growth Factors & Hormones; CDsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; CDs Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth

Applications Tested/Suitable for CD14 active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of CD14 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD14 purchase

MBS844753 is an active protein targeting CD14. Also known as CD-14, mouse recombinant. Host: CHO Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CD14. It may not necessarily be applicable to this product.

NCBI GI #

6753332

NCBI GeneID

12475

NCBI Accession #

NP_033971.1

UniProt Primary Accession #

P10810

UniProt Related Accession #

P10810

GenBank Nucleotide #

NM_009841.4

NCBI Official Full Name

monocyte differentiation antigen CD14

NCBI Official Synonym Full Names

CD14 antigen

NCBI Official Symbol

Cd14

UniProt Protein Name

Monocyte differentiation antigen CD14

UniProt Synonym Protein Names

Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14

Protein Family

Monocyte differentiation antigen

UniProt Gene Name

Cd14

UniProt Entry Name

CD14_MOUSE

NCBI Protein Information

monocyte differentiation antigen CD14

NCBI Summary for CD14

This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014]

UniProt Comments for CD14

CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Cellular Component: extracellular space; cell surface; membrane; plasma membrane; lipid raft

Molecular Function: lipopolysaccharide binding

Biological Process: immune system process; response to molecule of bacterial origin; innate immune response; positive regulation of endocytosis; inflammatory response; positive regulation of tumor necrosis factor production; positive regulation of cytokine secretion

Research Articles on CD14

1. Data show that guanine nucleotide-binding protein G(i) subunit alpha-1 and alpha-3 (Galphai1/3) can interact with CD14 antigen/Grb2-associated binding protein Gab1, which modulates macrophage polarization in vitro and in vivo.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD14 active protein

Reference Product	PubMed Publications
Tlr4 active protein	>1420 publications with CD14 and Tlr4
Itgam active protein	>744 publications with CD14 and Itgam
Tlr2 active protein	>742 publications with CD14 and Tlr2
Lbp active protein	>439 publications with CD14 and Lbp
Itgb2 active protein	>317 publications with CD14 and Itgb2
Ly96 active protein	>148 publications with CD14 and Ly96
Tlr6 active protein	>64 publications with CD14 and Tlr6
Ly86 active protein	>9 publications with CD14 and Ly86
Cd180 active protein	>8 publications with CD14 and Cd180

Pathways associated with CD14 active protein

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies	Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Caspase Activation Via Extrinsic Apoptotic Signalig Pathway antibodies	Caspase Activation Via Extrinsic Apoptotic Signalig Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
IKK Complex Recruitment Mediated By RIP1 Pathway antibodies	IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram

Diseases associated with CD14 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>2444 publications with CD14 and Inflammation
Necrosis Antibodies	>2361 publications with CD14 and Necrosis
Cardiovascular Diseases Antibodies	>685 publications with CD14 and Cardiovascular Diseases
Lung Diseases Antibodies	>559 publications with CD14 and Lung Diseases
Nervous System Diseases Antibodies	>377 publications with CD14 and Nervous System Diseases
Liver Diseases Antibodies	>373 publications with CD14 and Liver Diseases
Atherosclerosis Antibodies	>331 publications with CD14 and Atherosclerosis
Heart Diseases Antibodies	>293 publications with CD14 and Heart Diseases
Asthma Antibodies	>269 publications with CD14 and Asthma
Kidney Diseases Antibodies	>209 publications with CD14 and Kidney Diseases

Organs/Tissues associated with CD14 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>7756 publications with CD14 and Blood
Bone Antibodies	>1366 publications with CD14 and Bone
Bone Marrow Antibodies	>1094 publications with CD14 and Bone Marrow
Liver Antibodies	>638 publications with CD14 and Liver
Lung Antibodies	>615 publications with CD14 and Lung
Skin Antibodies	>425 publications with CD14 and Skin
Kidney Antibodies	>347 publications with CD14 and Kidney
Brain Antibodies	>317 publications with CD14 and Brain
Joint Antibodies	>292 publications with CD14 and Joint
Muscle Antibodies	>285 publications with CD14 and Muscle