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MIP-1alpha (CCL3) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844871

Background: Human microphage inflammatory protein-1 alpha (MIP-1alpha is a 7.8 kDa protein containing 70 amino acid residues. It plays an important role in the inflammatory response of blood monocytes and tissue microphages. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
MIP-1alpha (CCL3), Active Protein
Full Product Name
MIP-1alpha, human recombinant
Product Gene Name
CCL3 active protein
Product Synonym Names
Small inducible cytokine A3; CCL3; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; Tonsillar lymphocyte LD78 alpha protein; G0/G1 switch regulatory protein 19-1; G0S19-1 protein; SIS-beta; PAT 464,1; chemokine (C-C motif) ligand 3; MIP1A; SCYA3; G0S19-1; LD78alpha; TY-5; SIS-alpha; Heparin-binding chemotaxis protein; L2G25B;
Host
E Coli
Form/Format
Sterile filtered and then lyophilized with no additives
Appearance: Lyophilized protein
Purity/Purification
>=98%
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
7.8 kDa
OMIM
182283
3D Structure
ModBase 3D Structure for P10147
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Reconstitute in H?O to a concentration of 0.1-1.0 mg/ml. The solution can be diluted into other aqueous buffers or store at 4 degree C for 1 week and -20 degree C for future use.
Endotoxin Level
<0.1 ng/mug
Biological Activity
Activity was determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10 ng/ml.
Results
1.0-10 ng/ml
Handling
Centrifuge the vial prior to opening.

Product Categories/Family for CCL3 active protein

Cytokines; Growth Factors & Hormones; ChemokinesCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Chemokines Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytok
Applications Tested/Suitable for CCL3 active protein
SDS-PAGE; HPLC

Other Notes

Small volumes of CCL3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL3 purchase

MBS844871 is an active protein targeting CCL3. Also known as MIP-1alpha, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CCL3. It may not necessarily be applicable to this product.

NCBI GI #
4506843
NCBI GeneID
6348
NCBI Accession #
NP_002974.1
UniProt Primary Accession #
P10147
UniProt Related Accession #
P10147
GenBank Nucleotide #
NM_002983.2
NCBI Official Full Name
C-C motif chemokine 3
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 3
NCBI Official Symbol
CCL3
NCBI Official Synonym Symbols
MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
UniProt Protein Name
C-C motif chemokine 3
UniProt Synonym Protein Names
G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha
Protein Family
C-C motif chemokine
UniProt Gene Name
CCL3
UniProt Synonym Gene Names
G0S19-1; MIP1A; SCYA3; MIP-1-alpha
UniProt Entry Name
CCL3_HUMAN
NCBI Protein Information
C-C motif chemokine 3

NCBI Summary for CCL3

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]
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UniProt Comments for CCL3

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol

Molecular Function: identical protein binding; CCR1 chemokine receptor binding; protein binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; phospholipase activator activity; CCR5 chemokine receptor binding; chemoattractant activity; protein kinase activity

Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; response to toxin; behavior; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; monocyte chemotaxis; regulation of cell shape; negative regulation of bone mineralization; positive chemotaxis; cell-cell signaling; positive regulation of neuron apoptosis; calcium ion transport; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; calcium-mediated signaling; MAPKKK cascade; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; macrophage chemotaxis; osteoblast differentiation; cellular calcium ion homeostasis; cell activation; positive regulation of protein kinase B signaling cascade; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of inflammatory response; positive regulation of cell migration

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To
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Research Articles on CCL3

1. In COPD patients, CCL3 is upregulated in sputum and may facilitate recruitment of macrophages into airways.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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