TNFRSF10B Active Protein – Cat# MBS845305

Tnfrsf10b/TRAILR2 (TNFRSF10B) Active Protein

Name: TNFRSF10B Active Protein; Tnfrsf10b/TRAILR2; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS845305
Price: 310 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845305

Background: Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) also known as TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2), Death receptor 5 (DR5), CD262, KILLER, is a member of the TNF-receptor superfamily, and contains an… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TNFRSF10B/TRAILR2, Active Protein

Full Product Name

Human CellExp TNFRSF10B / TRAILR2, human recombinant

Product Gene Name

TNFRSF10B active protein

Product Synonym Names

TNFRSF10B; TRAILR2; TRAIL-R2; CD262; DR5; KILLER; TRICK2; ZTNFR9; TRICKB

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein fused with 6×His tag at the C-terminus, has a calculated MW of 15.1 kDa. The predicted N-terminus is Ile 56. DTT-reduced Protein migrates as 15-20 kDa due to glycosylation.

OMIM

275355

3D Structure

ModBase 3D Structure for O14763

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF10B at 10 mug/ml (100 mul/well) can bind biotinylated TNFSF10 with a linear range of 0.375 - 10 ng/ml.

Results

Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF10B at 10 mug/ml (100 mul/well) can bind biotinylated TNFSF10 with a linear range of 0.375 - 10 ng/ml.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for TNFRSF10B active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; TNFsProteins and Enzymes; Cytokines; Growth Factors &

Applications Tested/Suitable for TNFRSF10B active protein

SDS-PAGE

Other Notes

Small volumes of TNFRSF10B active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNFRSF10B purchase

MBS845305 is an active protein targeting TNFRSF10B. Also known as Human CellExp TNFRSF10B / TRAILR2, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for TNFRSF10B. It may not necessarily be applicable to this product.

NCBI GI #

224494019

NCBI GeneID

8795

NCBI Accession #

NP_003833.4

UniProt Primary Accession #

O14763

UniProt Secondary Accession #

O14720; O15508; O15517; O15531; Q6UXM8; Q7Z360; Q9BVE0

UniProt Related Accession #

O14763

GenBank Nucleotide #

NM_003842.4

NCBI Official Full Name

tumor necrosis factor receptor superfamily member 10B isoform 1

NCBI Official Synonym Full Names

tumor necrosis factor receptor superfamily, member 10b

NCBI Official Symbol

TNFRSF10B

NCBI Official Synonym Symbols

DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5

UniProt Protein Name

Tumor necrosis factor receptor superfamily member 10B

UniProt Synonym Protein Names

Death receptor 5; TNF-related apoptosis-inducing ligand receptor 2; TRAIL receptor 2; TRAIL-R2; CD_antigen: CD262

Protein Family

Tumor necrosis factor receptor superfamily

UniProt Gene Name

TNFRSF10B

UniProt Synonym Gene Names

DR5; KILLER; TRAILR2; TRICK2; ZTNFR9; TRAIL receptor 2; TRAIL-R2

UniProt Entry Name

TR10B_HUMAN

NCBI Protein Information

tumor necrosis factor receptor superfamily member 10B

NCBI Summary for TNFRSF10B

The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]

UniProt Comments for TNFRSF10B

TRAIL-R2: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B. Homotrimer. Can interact with TRADD and RIPK1. Regulated by p53/TP53. Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p22-p21

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; TRAIL binding; receptor activity

Biological Process: regulation of apoptosis; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; cell surface receptor linked signal transduction; induction of apoptosis via death domain receptors; apoptosis; activation of NF-kappaB-inducing kinase

Disease: Squamous Cell Carcinoma, Head And Neck

Research Articles on TNFRSF10B

1. Taken together, our results strongly suggest that FLLL12 induces apoptosis of lung cancer cell lines by posttranscriptional regulation of DR5 through activation of protein tyrosine phosphatase(s).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TNFRSF10B active protein

Reference Product	PubMed Publications
TNFSF10 active protein	>2881 publications with TNFRSF10B and TNFSF10
CASP8 active protein	>809 publications with TNFRSF10B and CASP8
TNFRSF10A active protein	>584 publications with TNFRSF10B and TNFRSF10A
FADD active protein	>426 publications with TNFRSF10B and FADD
CFLAR active protein	>158 publications with TNFRSF10B and CFLAR
TRADD active protein	>48 publications with TNFRSF10B and TRADD
CASP10 active protein	>36 publications with TNFRSF10B and CASP10
TP53 active protein	>35 publications with TNFRSF10B and TP53
TRAF2 active protein	>24 publications with TNFRSF10B and TRAF2
RIPK1 active protein	>22 publications with TNFRSF10B and RIPK1

Pathways associated with TNFRSF10B active protein

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
CASP8 Activity Is Inhibited Pathway antibodies	CASP8 Activity Is Inhibited Pathway Diagram
Caspase Activation Via Extrinsic Apoptotic Signalig Pathway antibodies	Caspase Activation Via Extrinsic Apoptotic Signalig Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
DNA Damage Response Pathway antibodies	DNA Damage Response Pathway Diagram

Diseases associated with TNFRSF10B active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>2116 publications with TNFRSF10B and Necrosis
Leukemia Antibodies	>263 publications with TNFRSF10B and Leukemia
Liver Diseases Antibodies	>178 publications with TNFRSF10B and Liver Diseases
Breast Neoplasms Antibodies	>173 publications with TNFRSF10B and Breast Neoplasms
Lung Neoplasms Antibodies	>162 publications with TNFRSF10B and Lung Neoplasms
Neoplasms, Experimental Antibodies	>157 publications with TNFRSF10B and Neoplasms, Experimental
Lung Diseases Antibodies	>153 publications with TNFRSF10B and Lung Diseases
Inflammation Antibodies	>145 publications with TNFRSF10B and Inflammation
Colonic Neoplasms Antibodies	>144 publications with TNFRSF10B and Colonic Neoplasms
Carcinoma, Hepatocellular Antibodies	>120 publications with TNFRSF10B and Carcinoma, Hepatocellular

Organs/Tissues associated with TNFRSF10B active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>502 publications with TNFRSF10B and Blood
Liver Antibodies	>236 publications with TNFRSF10B and Liver
Lung Antibodies	>218 publications with TNFRSF10B and Lung
Prostate Antibodies	>168 publications with TNFRSF10B and Prostate
Brain Antibodies	>108 publications with TNFRSF10B and Brain
Vascular Antibodies	>82 publications with TNFRSF10B and Vascular
Kidney Antibodies	>78 publications with TNFRSF10B and Kidney
Skin Antibodies	>69 publications with TNFRSF10B and Skin
Bone Marrow Antibodies	>61 publications with TNFRSF10B and Bone Marrow
Muscle Antibodies	>38 publications with TNFRSF10B and Muscle