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KDM4A Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS850571

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
KDM4A, Antibody
Full Product Name
KDM4A Antibody
Product Gene Name
Product Synonym Names
JMJD2; JHDM3A; JMJD2A; KIAA0677; KDM4A
Isotype
IgG1
Clone Number
5H1
Host
Mouse
Species Reactivity
Human
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.
Molecular Weight
120kDa
OMIM
609764
3D Structure
ModBase 3D Structure for O75164
Immunogen
Purified recombinant fragment of human KDM4A expressed in E. Coli.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-KDM4A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-KDM4A antibody
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-KDM4A antibody

Figure 1: Western blot analysis using KDM4A mAb against HEK293 (1) and KDM4A-hIgGFc transfected HEK293 (2) cell lysate.
Mouse Anti-KDM4A Antibody – Cat# MBS850571

Immunohistochemistry (IHC) of anti-KDM4A antibody

Figure 2: Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human larynx cancer tissues (right) using KDM4A mouse mAb with DAB staining.
Mouse Anti-KDM4A Antibody, Immunohistochemistry (IHC) – Cat# MBS850571

Immunofluorescence (IF) of anti-KDM4A antibody

Figure 3: Immunofluorescence analysis of NTERA-2 cells using KDM4A mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Mouse Anti-KDM4A Antibody, Immunofluorescence (IF) – Cat# MBS850571

Other Notes

Small volumes of anti-KDM4A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KDM4A purchase

MBS850571 is an antibody targeting KDM4A. Also known as KDM4A Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KDM4A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
lysine-specific demethylase 4A
NCBI Official Synonym Full Names
lysine demethylase 4A
NCBI Official Symbol
KDM4A
NCBI Official Synonym Symbols
JMJD2; JHDM3A; JMJD2A; TDRD14A
UniProt Protein Name
Lysine-specific demethylase 4A
UniProt Synonym Protein Names
JmjC domain-containing histone demethylation protein 3A; Jumonji domain-containing protein 2A
UniProt Gene Name
KDM4A
UniProt Synonym Gene Names
JHDM3A; JMJD2; JMJD2A; KIAA0677
NCBI Protein Information
lysine-specific demethylase 4A

UniProt Comments for KDM4A

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed:26741168). Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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