CCND3 Antibody – Mouse

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene.

Small volumes of anti-CCND3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

In the pathway to cancer, unregulated cell growth is an initial hallmark event. The cell cycle is a tightly regulated and coordinated interplay of specific cyclin (CCND) and kinase CDK interactions. Cyclin D3 CCND3 and it's kinase CDK4 are critical regulators in the signaling pathway leading to exit from G1 to S in a G1 specific cyclin manner. To facilitate research into this important biological process, a mouse monoclonal antibody is available for use in western blot or ELISA to detect human or mouse with cross species reactivity projected to be high based on CCND3 gene homology (rabbit or rat). This antibody is derived from a human recombinant protein from a custom E. coli expression cassette found in 1 publication CCND3 and is validated to detect proteins in the 10 ng range, showing significant improvement over rat CCND3 antibodies and more consistent antibody production for a quality product.