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MCM2 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS851553

MBS851553 is an antibody targeting MCM2. Also known as MCM2 Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MCM2, Antibody
Full Product Name
MCM2 Antibody
Product Gene Name
Product Synonym Names
BM28; CDCL1; DNA replication licensing factor MCM2; KIAA0030; MCMD2; Nuclear protein BM28
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
MCM2 antibody detects endogenous levels of total MCM2 protein.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
101,896 Da
OMIM
116945
3D Structure
ModBase 3D Structure for P49736
Immunogen
The antiserum was produced against synthesized peptide derived from human MCM2.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MCM2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-MCM2 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:40000

Immunohistochemistry (IHC) of anti-MCM2 antibody

Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MCM2 antibody.
Anti-MCM2 Antibody – Cat# MBS851553

Western Blot (WB) of anti-MCM2 antibody

Western blot analysis of extracts from 293 cells, using MCM2 antibody.
Anti-MCM2 Antibody, Western Blot (WB) – Cat# MBS851553

Immunofluorescence (IF) of anti-MCM2 antibody

Peptide-+ Immunofluorescence analysis of HepG2 cells, using MCM2 antibody.
Anti-MCM2 Antibody, Immunofluorescence (IF) – Cat# MBS851553

Product References and Citations for anti-MCM2 antibody

H Yan, Genes & Dev., Jun 1991; 5: 944-957. Yukio Ishimi, J. Biol. Chem., Nov 2001; 276: 42744-42752. Muluye E. Liku, Mol. Biol. Cell, Oct 2005; 16: 5026-5039. Daniela S. Dimitrova, J. Cell Biol., Aug 1999; 146: 709.

Other Notes

Small volumes of anti-MCM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MCM2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA replication licensing factor MCM2
NCBI Official Synonym Full Names
minichromosome maintenance complex component 2
NCBI Official Symbol
MCM2
NCBI Official Synonym Symbols
BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN
UniProt Protein Name
DNA replication licensing factor MCM2
UniProt Synonym Protein Names
Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
UniProt Gene Name
MCM2
UniProt Synonym Gene Names
BM28; CCNL1; CDCL1; KIAA0030
NCBI Protein Information
DNA replication licensing factor MCM2

NCBI Summary for MCM2

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]
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UniProt Comments for MCM2

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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