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NCK2 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS852501

MBS852501 is an antibody targeting NCK2. Also known as NCK2 Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NCK2, Antibody
Full Product Name
NCK2 Antibody
Product Gene Name
Product Synonym Names
Cytoplasmic protein NCK2; GRB4; NCK adaptor protein 2; NCKbeta; Nck-2; SH2/SH3 adaptor protein NCK-beta
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
NCK2 antibody detects endogenous levels of total NCK2 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
42,915 Da
OMIM
604930
3D Structure
ModBase 3D Structure for O43639
Immunogen
The antiserum was produced against synthesized peptide derived from C-terminal of human NCK2.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-NCK2 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-NCK2 antibody
IHC: 1:50-1:100
ELISA: 1:1000

Immunohistochemistry (IHC) of anti-NCK2 antibody

Peptide-+ Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using NCK2 antibody.
Anti-NCK2 Antibody – Cat# MBS852501

Product References and Citations for anti-NCK2 antibody

Susanne Frese, J. Biol. Chem., Jun 2006; 281: 18236-18245. Friedhelm Bladt, Mol. Cell. Biol., Jul 2003; 23: 4586-4597. Stephan J. Kempiak, J. Biol. Chem., Feb 2005; 280: 5836-5842. Min Chen, Mol. Cell. Biol., Nov 2000; 20: 7867-7880.

Other Notes

Small volumes of anti-NCK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NCK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cytoplasmic protein NCK2 isoform A
NCBI Official Synonym Full Names
NCK adaptor protein 2
NCBI Official Symbol
NCK2
NCBI Official Synonym Symbols
GRB4; NCKbeta
UniProt Protein Name
Cytoplasmic protein NCK2
UniProt Synonym Protein Names
Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; SH2/SH3 adaptor protein NCK-beta
Protein Family
UniProt Gene Name
NCK2
UniProt Synonym Gene Names
GRB4; Nck-2
NCBI Protein Information
cytoplasmic protein NCK2

NCBI Summary for NCK2

This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for NCK2

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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