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ATR Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS852945

MBS852945 is an antibody targeting ATR. Also known as ATR (Phospho-Ser428) Antibody. Reactive in Human. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATR, Antibody
Full Product Name
ATR (Phospho-Ser428) Antibody
Product Gene Name
Product Synonym Names
ataxia telangiectasia and Rad3-related protein; EC 2.7.11.1; FRAP-related protein; FRP1; kinase ATR; protein kinase ATR
Species Reactivity
Human
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
ATR (Phospho-Ser428) antibody detects endogenous levels of ATR only when phosphorylated at serine 428.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
297,479 Da
OMIM
210600
3D Structure
ModBase 3D Structure for Q13535
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ATR around the phosphorylation site of serine 428 (G-I-SP-P-K).
All Sites
Human: Ser428
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ATR antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-ATR antibody
IHC: 1:50-1:100
ELISA: 1:40000

Testing Data of anti-ATR antibody

P-peptide-+
Anti-ATR Antibody – Cat# MBS852945

Immunohistochemistry (IHC) of anti-ATR antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ATR (Phospho-Ser428) antibody. ATR (Phospho-Ser428) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.
Anti-ATR Antibody, Immunohistochemistry (IHC) – Cat# MBS852945

Product References and Citations for anti-ATR antibody

Bentley N.J., EMBO J. 15:6641-6651(1996). Cimprich K.A., Proc. Natl. Acad. Sci. U.S.A. 93:2850-2855(1996). Mannino J.L., Gene 272:35-43(2001).

Other Notes

Small volumes of anti-ATR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ATR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase ATR isoform 1
NCBI Official Synonym Full Names
ATR serine/threonine kinase
NCBI Official Symbol
ATR
NCBI Official Synonym Symbols
FRP1; MEC1; SCKL; FCTCS; SCKL1
UniProt Protein Name
Serine/threonine-protein kinase ATR
UniProt Synonym Protein Names
Ataxia telangiectasia and Rad3-related protein; FRAP-related protein 1
UniProt Gene Name
ATR
UniProt Synonym Gene Names
FRP1
NCBI Protein Information
serine/threonine-protein kinase ATR

NCBI Summary for ATR

The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1. [provided by RefSeq, Aug 2017]
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UniProt Comments for ATR

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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