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Calnexin (CANX) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS854351

MBS854351 is an antibody targeting CANX. Also known as Calnexin (Phospho-Ser583) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Calnexin (CANX), Antibody
Full Product Name
Calnexin (Phospho-Ser583) Antibody
Product Gene Name
Product Synonym Names
CALX; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
Calnexin (Phospho-Ser583) antibody detects endogenous levels of Calnexin only when phosphorylated at serine 583.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
55,598 Da
OMIM
114217
3D Structure
ModBase 3D Structure for P27824
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Calnexin around the phosphorylation site of serine 583 (N-R-SP-P-R).
All Sites
Human: Ser583
Mouse: Ser582
Rat: Ser582
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CANX antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-CANX antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000

Testing Data of anti-CANX antibody

P-peptide-+
Anti-CANX Antibody – Cat# MBS854351

Immunohistochemistry (IHC) of anti-CANX antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Calnexin (Phospho-Ser583) antibody. Western blot analysis of extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), using Calnexin (Phospho-Ser583) antibody.
Anti-CANX Antibody, Immunohistochemistry (IHC) – Cat# MBS854351

Immunofluorescence (IF) of anti-CANX antibody

P-peptide-+ Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) antibody.
Anti-CANX Antibody, Immunofluorescence (IF) – Cat# MBS854351

Product References and Citations for anti-CANX antibody

Shaun P Brothers, J. Mol. Endocrinol., Dec 2006; 37: 479-488. Vilasack Thammavongsa, J. Biol. Chem., Sep 2005; 280: 33497-33505. Yasushi Okazaki, J. Biol. Chem., Nov 2000; 275: 35751. Milka Popov, J. Biol. Chem., Jun 1999; 274: 17635.

Other Notes

Small volumes of anti-CANX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CANX. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
calnexin
NCBI Official Synonym Full Names
calnexin
NCBI Official Symbol
CANX
NCBI Official Synonym Symbols
CNX; P90; IP90
UniProt Protein Name
Calnexin
UniProt Synonym Protein Names
IP90; Major histocompatibility complex class I antigen-binding protein p88; p90
Protein Family
UniProt Gene Name
CANX
NCBI Protein Information
calnexin

NCBI Summary for CANX

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for CANX

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CANX antibody

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