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MAN1B1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS856412

MBS856412 is an antibody targeting MAN1B1. Also known as MAN1B1 Antibody. Reactive in Human. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAN1B1, Antibody
Full Product Name
MAN1B1 Antibody
Product Gene Name
Product Synonym Names
Endoplasmic reticulum mannosyl-oligosaccharide 1; 2-alpha-mannosidase; EC=3.2.1.113; ER alpha-1; 2-mannosidase; ER mannosidase 1; ERMan1; Man9GlcNAc2-specific-processing alpha-mannosidase; Mannosidase alpha class 1B member 1
Species Reactivity
Human
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
MAN1B1 antibody detects endogenous levels of total MAN1B1 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
79,580 Da
OMIM
604346
3D Structure
ModBase 3D Structure for Q9UKM7
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human MAN1B1.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MAN1B1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-MAN1B1 antibody
WB: 1:500-1:1000
ELISA: 1:1000

Western Blot (WB) of anti-MAN1B1 antibody

Western blot analysis of extracts from 3T3 cells, using MAN1B1 antibody.
Anti-MAN1B1 Antibody – Cat# MBS856412

Product References and Citations for anti-MAN1B1 antibody

Tremblay L.O., Glycobiology 9:1073-1078(1999) [PubMed: 10521544]. Gonzalez D.S., J. Biol. Chem. 274:21375-21386(1999) [PubMed: 10409699]. Clark H.F., Genome Res. 13:2265-2270(2003) [PubMed: 12975309]. Gonzalez D.S., J. Biol. Chem. 274:21375-21386(1999) [PubMed: 10409699]. Clark H.F., Genome Res. 13:2265-2270(2003) [PubMed: 12975309].

Other Notes

Small volumes of anti-MAN1B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAN1B1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
NCBI Official Synonym Full Names
mannosidase alpha class 1B member 1
NCBI Official Symbol
MAN1B1
NCBI Official Synonym Symbols
MRT15; ERMAN1; ERManI; MANA-ER
UniProt Protein Name
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
UniProt Synonym Protein Names
ER alpha-1,2-mannosidase; ER mannosidase 1; ERMan1; Man9GlcNAc2-specific-processing alpha-mannosidase; Mannosidase alpha class 1B member 1
UniProt Gene Name
MAN1B1
UniProt Synonym Gene Names
ERMan1
NCBI Protein Information
endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

NCBI Summary for MAN1B1

This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Dec 2011]
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UniProt Comments for MAN1B1

Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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