Interleukin 7 (IL-7) ELISA Kit for Cat

Name: Cat IL-7 ELISA Kit; Interleukin 7; MyBioSource
Brand: MyBioSource
SKU: MBS086204
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS086204

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated IL-7 concentrations in Cat serum, plasma and other body fluids. Using Purified Cat IL-7 antibody to coat Microelisa Stripplate wells to… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Interleukin 7 (IL-7), ELISA Kit

Full Product Name

Cat Interleukin 7 ELISA Kit

Product Gene Name

IL-7 elisa kit

Species Reactivity

Cat

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces and Urine

Detection Range

6.25 pg/ml - 200 pg/ml.

Sensitivity

1.0 pg/ml.

Specificity

No significant cross-reactivity or interference between Cat IL-7 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

20,187 Da

Chromosome Location

Chromosome: 8; NC_000008.10 (79645007..79717758, complement). Location: 8q12-q13

OMIM

146660

3D Structure

ModBase 3D Structure for P13232

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated IL-7 concentrations in Cat serum, plasma and other body fluids. Using Purified Cat IL-7 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-7 and IL-7 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of IL-7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-7 purchase

MBS086204 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 7 (IL-7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-7. The ELISA analytical biochemical technique of the MBS086204 kit is based on IL-7 antibody-IL-7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IL-7. It may not necessarily be applicable to this product.

NCBI GI #

315467871

NCBI GeneID

3574

NCBI Accession #

NP_001186817.1

UniProt Primary Accession #

P13232

UniProt Secondary Accession #

Q5FBY5; Q5FBY9; A0N0L3

UniProt Related Accession #

P13232

GenBank Nucleotide #

NM_001199888.1

NCBI Official Full Name

interleukin-7 isoform 4

NCBI Official Synonym Full Names

interleukin 7

NCBI Official Symbol

IL7

NCBI Official Synonym Symbols

IL-7

UniProt Protein Name

Interleukin-7

Protein Family

Interleukin-7 receptor

UniProt Gene Name

IL7

UniProt Synonym Gene Names

IL-7

UniProt Entry Name

IL7_HUMAN

NCBI Protein Information

interleukin-7

NCBI Summary for IL-7

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]

UniProt Comments for IL-7

IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell development/differentiation; Cell cycle regulation; Secreted, signal peptide; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 8q12-q13

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-7 receptor binding; protein binding; growth factor activity; cytokine activity

Biological Process: T cell lineage commitment; organ morphogenesis; cell-cell signaling; positive regulation of T cell differentiation; regulation of gene expression; homeostasis of number of cells within a tissue; negative regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of organ growth; humoral immune response; bone resorption; negative regulation of apoptosis

Research Articles on IL-7

1. Increase in the level of IL-7 is associated with skin pathogenesis in breast cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL-7 elisa kit

Reference Product	PubMed Publications
IL7R elisa kit	>11 publications with IL-7 and IL7R
IL2RG elisa kit	>9 publications with IL-7 and IL2RG
CD19 elisa kit	>8 publications with IL-7 and CD19
IL3 elisa kit	>8 publications with IL-7 and IL3
JAK3 elisa kit	>8 publications with IL-7 and JAK3
IL2RA elisa kit	>7 publications with IL-7 and IL2RA
IL9 elisa kit	>7 publications with IL-7 and IL9
CSF2 elisa kit	>3 publications with IL-7 and CSF2
JAK1 elisa kit	>2 publications with IL-7 and JAK1

Pathways associated with IL-7 elisa kit

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Interleukin-7 Signaling Pathway antibodies	Interleukin-7 Signaling Pathway Diagram
Interleukin-7 Signaling Pathway antibodies	Interleukin-7 Signaling Pathway Diagram

Diseases associated with IL-7 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>59 publications with IL-7 and Neoplasms
Inflammation Antibodies	>19 publications with IL-7 and Inflammation
Necrosis Antibodies	>18 publications with IL-7 and Necrosis
Lymphopenia Antibodies	>17 publications with IL-7 and Lymphopenia
Nervous System Diseases Antibodies	>16 publications with IL-7 and Nervous System Diseases
Multiple Sclerosis Antibodies	>15 publications with IL-7 and Multiple Sclerosis
Neoplasms, Experimental Antibodies	>14 publications with IL-7 and Neoplasms, Experimental
Carcinoma Antibodies	>9 publications with IL-7 and Carcinoma
Hypersensitivity Antibodies	>6 publications with IL-7 and Hypersensitivity
Cell Transformation, Neoplastic Antibodies	>6 publications with IL-7 and Cell Transformation, Neoplastic

Organs/Tissues associated with IL-7 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>180 publications with IL-7 and Blood
Bone Antibodies	>45 publications with IL-7 and Bone
Thymus Antibodies	>36 publications with IL-7 and Thymus
Lymph Node Antibodies	>19 publications with IL-7 and Lymph Node
Skin Antibodies	>11 publications with IL-7 and Skin
Spleen Antibodies	>10 publications with IL-7 and Spleen
Liver Antibodies	>9 publications with IL-7 and Liver
Brain Antibodies	>7 publications with IL-7 and Brain
Intestine Antibodies	>5 publications with IL-7 and Intestine
Kidney Antibodies	>3 publications with IL-7 and Kidney