Alpha-2-Macroglobulin (A2M) ELISA Kit for Mouse

Name: Mouse A2M ELISA Kit; Alpha-2-Macroglobulin; MyBioSource
Brand: MyBioSource
SKU: MBS900336
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS900336

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with A2M. Standards or samples are then added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for A2M,… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

alpha-2-macroglobulin (A2M), ELISA Kit

Full Product Name

Mouse Alpha-2-macroglobulin, A2M ELISA Kit

Product Gene Name

A2M elisa kit

Product Synonym Names

Mouse Alpha-2-macroglobulin (A2M) ELISA kit; CPAMD5; DKFZp779B086; FWP007; S863-7; ; alpha-2-macroglobulin

Species Reactivity

Mouse

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse A2M. No significant cross-reactivity or interference between Mouse A2M and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

165,853 Da

3D Structure

ModBase 3D Structure for Q61838

Detection Wavelength

450 nm

Sample Volume

50-100ul

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of A2M elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for A2M purchase

MBS900336 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-2-macroglobulin (A2M) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing A2M. The ELISA analytical biochemical technique of the MBS900336 kit is based on A2M antibody-A2M antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect A2M antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, A2M. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for A2M. It may not necessarily be applicable to this product.

NCBI GI #

110347469

NCBI GeneID

11287

NCBI Accession #

NP_031402.3

UniProt Primary Accession #

Q61838

UniProt Secondary Accession #

Q60628; Q6PEM2; E9QPW0

UniProt Related Accession #

Q61838

GenBank Nucleotide #

NM_007376.4

NCBI Official Full Name

alpha-2-macroglobulin

NCBI Official Synonym Full Names

pregnancy zone protein

NCBI Official Symbol

Pzp

NCBI Official Synonym Symbols

A1m; A2m; MAM; AI893533

UniProt Protein Name

Alpha-2-macroglobulin

UniProt Synonym Protein Names

Pregnancy zone proteinCleaved into the following 2 chains:Alpha-2-macroglobulin 165 kDa subunit; Alpha-2-macroglobulin 35 kDa subunit

Protein Family

Alpha-2-macroglobulin

UniProt Gene Name

A2m

UniProt Synonym Gene Names

Pzp; Alpha-2-M

UniProt Entry Name

A2M_MOUSE

NCBI Protein Information

alpha-2-macroglobulin; alpha-2-M; alpha 1 macroglobulin

UniProt Comments for A2M

PZP: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protease inhibitor activity; endopeptidase inhibitor activity; protein complex binding

Biological Process: negative regulation of peptidase activity; embryo implantation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with A2M elisa kit

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>3 publications with A2M and Fatty Liver
Brain Diseases Antibodies	>2 publications with A2M and Brain Diseases
Inflammation Antibodies	>2 publications with A2M and Inflammation
Weight Loss Antibodies	>2 publications with A2M and Weight Loss
Body Weight Changes Antibodies	>2 publications with A2M and Body Weight Changes
Fibrosis Antibodies	>1 publications with A2M and Fibrosis
Urinary Bladder Neoplasms Antibodies	>1 publications with A2M and Urinary Bladder Neoplasms
Liver Diseases Antibodies	>1 publications with A2M and Liver Diseases
Liver Neoplasms Antibodies	>1 publications with A2M and Liver Neoplasms
Prostatic Diseases Antibodies	>1 publications with A2M and Prostatic Diseases

Organs/Tissues associated with A2M elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>11 publications with A2M and Brain
Embryonic Tissue Antibodies	>10 publications with A2M and Embryonic Tissue
Liver Antibodies	>9 publications with A2M and Liver
Muscle Antibodies	>4 publications with A2M and Muscle
Kidney Antibodies	>2 publications with A2M and Kidney
Lung Antibodies	>1 publications with A2M and Lung