Triosephosphate Isomerase 1 (TPI1) ELISA Kit for Human

Name: Human TPI1 ELISA Kit; Triosephosphate Isomerase 1; MyBioSource
Brand: MyBioSource
SKU: MBS901437
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS901437

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPI1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

triosephosphate isomerase 1 (TPI1), ELISA Kit

Full Product Name

Human Triosephosphate isomerase, TPI1 ELISA Kit

Product Gene Name

TPI1 elisa kit

Product Synonym Names

Human Triosephosphate isomerase (TPI1) ELISA kit; MGC88108; TPI; ; triosephosphate isomerase 1

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates

Detection Range

28 pg/ml-1800 pg/ml

Sensitivity

< 7 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human TPI1. No significant cross-reactivity or interference between human TPI1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

30,791 Da

OMIM

gene 615512

3D Structure

ModBase 3D Structure for P60174

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of TPI1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPI1 purchase

MBS901437 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the triosephosphate isomerase 1 (TPI1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TPI1. The ELISA analytical biochemical technique of the MBS901437 kit is based on TPI1 antibody-TPI1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPI1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPI1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TPI1. It may not necessarily be applicable to this product.

NCBI GI #

4507645

NCBI GeneID

7167

NCBI Accession #

NP_000356.1

UniProt Primary Accession #

P60174

UniProt Secondary Accession #

P00938; Q6FHP9; Q6IS07; Q8WWD0; Q96AG5; B7Z5D8; D3DUS9

UniProt Related Accession #

P60174

GenBank Nucleotide #

NM_000365.5

NCBI Official Full Name

triosephosphate isomerase isoform 1

NCBI Official Synonym Full Names

triosephosphate isomerase 1

NCBI Official Symbol

TPI1

NCBI Official Synonym Symbols

TIM; TPI; TPID; HEL-S-49

UniProt Protein Name

Triosephosphate isomerase

UniProt Synonym Protein Names

Triose-phosphate isomerase

Protein Family

Triosephosphate isomerase

UniProt Gene Name

TPI1

UniProt Synonym Gene Names

TPI; TIM

UniProt Entry Name

TPIS_HUMAN

NCBI Protein Information

triosephosphate isomerase; triose-phosphate isomerase; epididymis secretory protein Li 49

NCBI Summary for TPI1

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

UniProt Comments for TPI1

TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 5.3.1.1; Isomerase; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: extracellular space; cytosol; nucleus

Molecular Function: protein binding; triose-phosphate isomerase activity

Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis

Disease: Triosephosphate Isomerase Deficiency

Research Articles on TPI1

1. E104D mutant is highly susceptible to proteolysis, which in all likelihood contributes to the pathogenesis of enzymopathy. In addition, the proteolysis data on wild type HsTIM illustrate an asymmetric conduct of the two monomers.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with TPI1 elisa kit

Products by Pathway	Pathway Diagram
Fatty Acid Beta Oxidation Pathway antibodies	Fatty Acid Beta Oxidation Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Gluconeogenesis Pathway antibodies	Gluconeogenesis Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Glucose Metabolism Pathway antibodies	Glucose Metabolism Pathway Diagram
Glycolysis Pathway antibodies	Glycolysis Pathway Diagram
Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies	Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies	Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram

Diseases associated with TPI1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>7 publications with TPI1 and Neoplasms
Breast Neoplasms Antibodies	>3 publications with TPI1 and Breast Neoplasms
Skin Diseases Antibodies	>3 publications with TPI1 and Skin Diseases
Disease Models, Animal Antibodies	>3 publications with TPI1 and Disease Models, Animal
Adenocarcinoma Antibodies	>3 publications with TPI1 and Adenocarcinoma
Hypertension Antibodies	>2 publications with TPI1 and Hypertension
Drug Toxicity Antibodies	>1 publications with TPI1 and Drug Toxicity
Liver Neoplasms Antibodies	>1 publications with TPI1 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with TPI1 and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>1 publications with TPI1 and Liver Diseases

Organs/Tissues associated with TPI1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5 publications with TPI1 and Blood
Brain Antibodies	>3 publications with TPI1 and Brain
Prostate Antibodies	>2 publications with TPI1 and Prostate
Muscle Antibodies	>2 publications with TPI1 and Muscle
Kidney Antibodies	>2 publications with TPI1 and Kidney
Mouth Antibodies	>1 publications with TPI1 and Mouth
Connective Tissue Antibodies	>1 publications with TPI1 and Connective Tissue
Heart Antibodies	>1 publications with TPI1 and Heart
Liver Antibodies	>1 publications with TPI1 and Liver
Adipose Tissue Antibodies	>1 publications with TPI1 and Adipose Tissue