Apolipoprotein A-IV (APOA4) ELISA Kit for Rat

Name: Rat APOA4 ELISA Kit; Apolipoprotein A-IV; MyBioSource
Brand: MyBioSource
SKU: MBS902667
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS902667

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APOA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOA4 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

apolipoprotein A-IV (APOA4), ELISA Kit

Full Product Name

Rat Apolipoprotein A-IV, APOA4 ELISA Kit

Product Gene Name

APOA4 elisa kit

Product Synonym Names

Rat Apolipoprotein A-IV (APOA4) ELISA kit; MGC142154; MGC142156; ; apolipoprotein A-IV

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates.

Detection Range

9.38 ng/ml -600 ng/ml.

Sensitivity

2.34 ng/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of rat APOA4. No significant cross-reactivity or interference between rat APOA4 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

44,456 Da

3D Structure

ModBase 3D Structure for P02651

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of APOA4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APOA4 purchase

MBS902667 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apolipoprotein A-IV (APOA4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOA4. The ELISA analytical biochemical technique of the MBS902667 kit is based on APOA4 antibody-APOA4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOA4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOA4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for APOA4. It may not necessarily be applicable to this product.

NCBI GI #

8392909

NCBI GeneID

25080

NCBI Accession #

NP_036869.1

UniProt Primary Accession #

P02651

UniProt Secondary Accession #

Q5BK92

UniProt Related Accession #

P02651

GenBank Nucleotide #

NM_012737.1

NCBI Official Full Name

apolipoprotein A-IV

NCBI Official Synonym Full Names

apolipoprotein A-IV

NCBI Official Symbol

Apoa4

NCBI Official Synonym Symbols

apoAIV; Apo-AIV; ApoA-IV

UniProt Protein Name

Apolipoprotein A-IV

UniProt Synonym Protein Names

Apolipoprotein A4

Protein Family

Apolipoprotein

UniProt Gene Name

Apoa4

UniProt Synonym Gene Names

Apo-AIV; ApoA-IV

UniProt Entry Name

APOA4_RAT

NCBI Protein Information

apolipoprotein A-IV; apolipoprotein A4

NCBI Summary for APOA4

may play a role in lipid transport [RGD, Feb 2006]

UniProt Comments for APOA4

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Endoplasmic reticulum; Lipid-binding; Secreted, signal peptide; Secreted; Cell adhesion

Cellular Component: extracellular space; chylomicron; cell surface

Molecular Function: antioxidant activity; protein homodimerization activity; copper ion binding; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: response to food; cholesterol metabolic process; multicellular organismal lipid catabolic process; positive regulation of fatty acid biosynthetic process; removal of superoxide radicals; lipid homeostasis; protein-lipid complex assembly; lipoprotein metabolic process; cholesterol efflux; lipid transport; cholesterol biosynthetic process; axon regeneration in the peripheral nervous system; phosphatidylcholine metabolic process; leukocyte adhesion; cholesterol homeostasis; reverse cholesterol transport; regulation of cholesterol absorption; phospholipid efflux; innate immune response in mucosa; hydrogen peroxide catabolic process; response to triglyceride; response to lipid hydroperoxide; positive regulation of lipoprotein lipase activity; regulation of cholesterol transport

Research Articles on APOA4

1. Data suggest that apoAIV in NTS (nucleus of the solitary tract) or/and peripheral Cck (cholecystokinin) require vagal Cck1r (cholecystokinin receptor 1) signaling to elicit satiation; high-fat diet reduces satiating capacity of these signals.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with APOA4 elisa kit

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Diseases associated with APOA4 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>22 publications with APOA4 and Cardiovascular Diseases
Heart Diseases Antibodies	>14 publications with APOA4 and Heart Diseases
Myocardial Ischemia Antibodies	>13 publications with APOA4 and Myocardial Ischemia
Dyslipidemias Antibodies	>12 publications with APOA4 and Dyslipidemias
Hyperlipidemias Antibodies	>11 publications with APOA4 and Hyperlipidemias
Atherosclerosis Antibodies	>10 publications with APOA4 and Atherosclerosis
Hypertriglyceridemia Antibodies	>8 publications with APOA4 and Hypertriglyceridemia
Inflammation Antibodies	>7 publications with APOA4 and Inflammation
Arteriosclerosis Antibodies	>7 publications with APOA4 and Arteriosclerosis
Brain Diseases Antibodies	>3 publications with APOA4 and Brain Diseases

Organs/Tissues associated with APOA4 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>14 publications with APOA4 and Liver
Small Intestine Antibodies	>2 publications with APOA4 and Small Intestine