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Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS908147

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for vWF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any vWF present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
wingless-type MMTV integration site family, member 1 (WNT1), ELISA Kit
Full Product Name
Mouse Proto-oncogene Wnt-1, WNT1 ELISA Kit
Product Gene Name
WNT1 elisa kit
Product Synonym Names
Mouse Proto-oncogene Wnt-1 (WNT1) ELISA kit; INT1; Wingless-type MMTV integration site family; member 1 (oncogene INT1) ; wingless-type MMTV integration site family; member 1
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Samples
Serum, Plasma, Tissue Homogenates
Detection Range
6.25 ng/ml-400 ng/ml
Sensitivity
1.56 ng/ml.
Specificity
This assay has high sensitivity and excellent specificity for detection of human vWF. No significant cross-reactivity or interference between human vWF and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Molecular Weight
41,086 Da
3D Structure
ModBase 3D Structure for P04426
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of WNT1 elisa kit

Mouse WNT1 ELISA Kit – Cat# MBS908147

Other Notes

Small volumes of WNT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WNT1 purchase

MBS908147 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the wingless-type MMTV integration site family, member 1 (WNT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT1. The ELISA analytical biochemical technique of the MBS908147 kit is based on WNT1 antibody-WNT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for WNT1. It may not necessarily be applicable to this product.

NCBI GI #
33469103
NCBI GeneID
22408
NCBI Accession #
NP_067254.1
UniProt Primary Accession #
P04426
UniProt Related Accession #
P04426
GenBank Nucleotide #
NM_021279.4
NCBI Official Full Name
proto-oncogene Wnt-1
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 1
NCBI Official Symbol
Wnt1
NCBI Official Synonym Symbols
sw; Int-1; Wnt-1; swaying
UniProt Protein Name
Proto-oncogene Wnt-1
UniProt Synonym Protein Names
Proto-oncogene Int-1
Protein Family
Proto-oncogene
UniProt Gene Name
Wnt1
UniProt Synonym Gene Names
Int-1; Wnt-1
UniProt Entry Name
WNT1_MOUSE
NCBI Protein Information
proto-oncogene Wnt-1; proto-oncogene Int-1; proto-oncogene protein Wnt-1; wingless-related MMTV integration site 1

UniProt Comments for WNT1

WNT1: Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted; Cell development/differentiation; Motility/polarity/chemotaxis; Secreted, signal peptide; Oncoprotein

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; cytoplasm; extracellular region

Molecular Function: protein domain specific binding; protein binding; frizzled binding; cytokine activity; receptor binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; multicellular organismal development; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; ubiquitin-dependent SMAD protein catabolic process; signal transduction; diencephalon development; negative regulation of BMP signaling pathway; BMP signaling pathway; cerebellum formation; positive regulation of fibroblast proliferation; cell-cell signaling; positive regulation of cell proliferation; midbrain development; midbrain-hindbrain boundary maturation during brain development; myoblast fusion; positive regulation of Notch signaling pathway; neuron fate determination; organ regeneration; inner ear morphogenesis; Wnt receptor signaling pathway; embryonic axis specification; neuron fate commitment; DNA damage response, signal transduction; myotube differentiation; negative regulation of fat cell differentiation; metencephalon development; organ morphogenesis; negative regulation of cell differentiation; central nervous system morphogenesis; ureteric bud branching; forebrain anterior/posterior pattern formation; midbrain-hindbrain boundary development; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell-cell adhesion; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; Spemann organizer formation
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Research Articles on WNT1

1. a broader role for Wnt/beta-catenin signaling in contributing to progenitor cell proliferation in nonhairy epithelia and interfollicular epidermis under homeostasis, is reported.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with WNT1 elisa kit

 Reference Product  PubMed Publications
 Porcn elisa kit  >3 publications with WNT1 and Porcn

Pathways associated with WNT1 elisa kit

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Dopminergic Neurogenesis Pathway antibodies  Dopminergic Neurogenesis Pathway Diagram
 ESC Pluripotency Pathways antibodies  ESC Pluripotency Pathways Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with WNT1 elisa kit

 Disease Name  Pubmed Publications
 Death Antibodies  >78 publications with WNT1 and Death
 Abnormalities, Multiple Antibodies  >29 publications with WNT1 and Abnormalities, Multiple
 Brain Diseases Antibodies  >26 publications with WNT1 and Brain Diseases
 Craniofacial Abnormalities Antibodies  >24 publications with WNT1 and Craniofacial Abnormalities
 Lung Diseases Antibodies  >22 publications with WNT1 and Lung Diseases
 Musculoskeletal Abnormalities Antibodies  >21 publications with WNT1 and Musculoskeletal Abnormalities
 Liver Diseases Antibodies  >20 publications with WNT1 and Liver Diseases
 Fibrosis Antibodies  >18 publications with WNT1 and Fibrosis
 Neural Tube Defects Antibodies  >15 publications with WNT1 and Neural Tube Defects
 Inflammation Antibodies  >14 publications with WNT1 and Inflammation

Organs/Tissues associated with WNT1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >298 publications with WNT1 and Embryonic Tissue
 Lung Antibodies  >40 publications with WNT1 and Lung
 Uncharacterized Tissue Antibodies  >2 publications with WNT1 and Uncharacterized Tissue
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