Rat NT5E/CD73 ELISA Kit – Cat# MBS908567

5'-Nucleotidase/Cluster of Differentiation 73 (NT5E/CD73) (NT5E/CD73) ELISA Kit for Rat

Name: Rat NT5E/CD73 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS908567
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS908567

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NT5E/CD73 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NT5E/CD73 present is bound by… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73), ELISA Kit

Full Product Name

Rat 5'-nucleotidase/cluster of differentiation 73, NT5E/CD73 ELISA Kit

Product Gene Name

NT5E/CD73 elisa kit

Product Synonym Names

Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit; 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73)

Species Reactivity

Rat

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell culture supernates

Detection Range

0.625 mIU/ml-40 mIU/ml

Sensitivity

< 0.156 mIU/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of rat NT5E/CD73. No significant cross-reactivity or interference between rat NT5E/CD73 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%.Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

28,841 Da

3D Structure

ModBase 3D Structure for Q96B60

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NT5E/CD73 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NT5E/CD73 purchase

MBS908567 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NT5E/CD73. The ELISA analytical biochemical technique of the MBS908567 kit is based on NT5E/CD73 antibody-NT5E/CD73 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NT5E/CD73 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NT5E/CD73. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NT5E/CD73. It may not necessarily be applicable to this product.

NCBI GI #

16198518

NCBI GeneID

4907

NCBI Accession #

AAH15940.1

UniProt Primary Accession #

Q96B60

UniProt Related Accession #

P21589

NCBI Official Full Name

NT5E protein

NCBI Official Synonym Full Names

5'-nucleotidase, ecto (CD73)

NCBI Official Symbol

NT5E

NCBI Official Synonym Symbols

NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA

UniProt Protein Name

5'-nucleotidase

UniProt Synonym Protein Names

5'-nucleotidase; 5'-nucleotidase, ecto (CD73), isoform CRA_c; NT5E protein

UniProt Gene Name

NT5E

UniProt Synonym Gene Names

hCG_401111

UniProt Entry Name

Q96B60_HUMAN

NCBI Protein Information

5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase

NCBI Summary for NT5E/CD73

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

UniProt Comments for NT5E/CD73

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Membrane protein, integral; EC 3.1.3.5; Nucleotide Metabolism - pyrimidine; Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries

Research Articles on NT5E/CD73

1. Isoflurane causes TGF-beta1-dependent induction of renal tubular CD73 and adenosine generation to protect against renal ischemia and reperfusion injury.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with NT5E/CD73 elisa kit

Products by Pathway	Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Nicotinate And Nicotinamide Metabolism Pathway antibodies	Nicotinate And Nicotinamide Metabolism Pathway Diagram
Nicotinate And Nicotinamide Metabolism Pathway antibodies	Nicotinate And Nicotinamide Metabolism Pathway Diagram
Purine Catabolism Pathway antibodies	Purine Catabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Pyrimidine Catabolism Pathway antibodies	Pyrimidine Catabolism Pathway Diagram

Diseases associated with NT5E/CD73 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>12 publications with NT5E/CD73 and Neoplasms
Nervous System Diseases Antibodies	>6 publications with NT5E/CD73 and Nervous System Diseases
Pain Antibodies	>5 publications with NT5E/CD73 and Pain
Inflammation Antibodies	>5 publications with NT5E/CD73 and Inflammation
Breast Neoplasms Antibodies	>3 publications with NT5E/CD73 and Breast Neoplasms
Gastrointestinal Diseases Antibodies	>3 publications with NT5E/CD73 and Gastrointestinal Diseases
Immune System Diseases Antibodies	>3 publications with NT5E/CD73 and Immune System Diseases
Disease Models, Animal Antibodies	>2 publications with NT5E/CD73 and Disease Models, Animal
Glioma Antibodies	>2 publications with NT5E/CD73 and Glioma
Ischemia Antibodies	>2 publications with NT5E/CD73 and Ischemia

Organs/Tissues associated with NT5E/CD73 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>11 publications with NT5E/CD73 and Blood
Vascular Antibodies	>8 publications with NT5E/CD73 and Vascular
Brain Antibodies	>5 publications with NT5E/CD73 and Brain
Ganglia Antibodies	>4 publications with NT5E/CD73 and Ganglia
Muscle Antibodies	>3 publications with NT5E/CD73 and Muscle
Mouth Antibodies	>3 publications with NT5E/CD73 and Mouth
Skin Antibodies	>3 publications with NT5E/CD73 and Skin
Intestine Antibodies	>2 publications with NT5E/CD73 and Intestine
Embryonic Tissue Antibodies	>2 publications with NT5E/CD73 and Embryonic Tissue
Bone Antibodies	>2 publications with NT5E/CD73 and Bone