CD47 Molecule (CD47) ELISA Kit for Mouse

Name: Mouse CD47 ELISA Kit; CD47 Molecule; MyBioSource
Brand: MyBioSource
SKU: MBS910495
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS910495

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD47 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD47 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

CD47 molecule, ELISA Kit

Full Product Name

Mouse Leukocyte surface antigen CD47, CD47 ELISA Kit

Product Gene Name

CD47 elisa kit

Product Synonym Names

Mouse Leukocyte surface antigen CD47 (CD47) ELISA kit; IAP; MER6; OA3; CD47 antigen; CD47 antigen (Rh-related antigen; integrin-associated signal transducer) ; CD47 glycoprotein; Rh-related antigen; antigen identified by monoclonal antibody 1D8; antigenic sur; CD47 molecule

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

15.6 pg/ml-1000 pg/ml

Sensitivity

< 3.9 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse CD47. No significant cross-reactivity or interference between mouse CD47 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%.Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

33,098 Da

3D Structure

ModBase 3D Structure for Q61735

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CD47 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD47 purchase

MBS910495 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD47 molecule, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD47. The ELISA analytical biochemical technique of the MBS910495 kit is based on CD47 antibody-CD47 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD47 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD47. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CD47. It may not necessarily be applicable to this product.

NCBI GI #

6754382

NCBI GeneID

16423

NCBI Accession #

NP_034711.1

UniProt Primary Accession #

Q61735

UniProt Secondary Accession #

Q921Z2

UniProt Related Accession #

Q61735

GenBank Nucleotide #

NM_010581.3

NCBI Official Full Name

leukocyte surface antigen CD47

NCBI Official Synonym Full Names

CD47 antigen (Rh-related antigen, integrin-associated signal transducer)

NCBI Official Symbol

Cd47

NCBI Official Synonym Symbols

IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik

UniProt Protein Name

Leukocyte surface antigen CD47

UniProt Synonym Protein Names

Integrin-associated protein; IAP; CD_antigen: CD47

Protein Family

Leukocyte surface antigen

UniProt Gene Name

Cd47

UniProt Synonym Gene Names

IAP

UniProt Entry Name

CD47_MOUSE

NCBI Protein Information

leukocyte surface antigen CD47; Rh-related antigen; integrin-associated protein

UniProt Comments for CD47

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: cell migration; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; opsonization; positive regulation of T cell activation; signal transduction; cell adhesion; positive regulation of inflammatory response

Research Articles on CD47

1. sought to investigate the effect of Hsp70-peptide complex on the expression of CD172alpha and CD47 receptors in normal peritoneal macrophages (NMO)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD47 elisa kit

Reference Product	PubMed Publications
Thbs1 elisa kit	>140 publications with CD47 and Thbs1
Sirpa elisa kit	>69 publications with CD47 and Sirpa
Ptpn6 elisa kit	>7 publications with CD47 and Ptpn6
Comp elisa kit	>2 publications with CD47 and Comp
Grb2 elisa kit	>1 publications with CD47 and Grb2
Ptk2b elisa kit	>1 publications with CD47 and Ptk2b
Thbs2 elisa kit	>1 publications with CD47 and Thbs2

Pathways associated with CD47 elisa kit

Products by Pathway	Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Cell-Cell Communication Pathway antibodies	Cell-Cell Communication Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Integrin Cell Surface Interactions Pathway antibodies	Integrin Cell Surface Interactions Pathway Diagram
Signal Regulatory Protein (SIRP) Family Interactions Pathway antibodies	Signal Regulatory Protein (SIRP) Family Interactions Pathway Diagram
Spinal Cord Injury Pathway antibodies	Spinal Cord Injury Pathway Diagram
XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies	XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram

Diseases associated with CD47 elisa kit

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>82 publications with CD47 and Immune System Diseases
Inflammation Antibodies	>77 publications with CD47 and Inflammation
Disease Models, Animal Antibodies	>44 publications with CD47 and Disease Models, Animal
Anemia Antibodies	>43 publications with CD47 and Anemia
Necrosis Antibodies	>40 publications with CD47 and Necrosis
Nervous System Diseases Antibodies	>32 publications with CD47 and Nervous System Diseases
Brain Diseases Antibodies	>27 publications with CD47 and Brain Diseases
Neoplasms, Experimental Antibodies	>21 publications with CD47 and Neoplasms, Experimental
Respiratory Tract Diseases Antibodies	>14 publications with CD47 and Respiratory Tract Diseases
Breast Neoplasms Antibodies	>10 publications with CD47 and Breast Neoplasms

Organs/Tissues associated with CD47 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>333 publications with CD47 and Blood
Bone Antibodies	>53 publications with CD47 and Bone
Muscle Antibodies	>43 publications with CD47 and Muscle
Brain Antibodies	>41 publications with CD47 and Brain
Skin Antibodies	>22 publications with CD47 and Skin
Spleen Antibodies	>22 publications with CD47 and Spleen
Lung Antibodies	>19 publications with CD47 and Lung
Liver Antibodies	>17 publications with CD47 and Liver
Lymph Node Antibodies	>14 publications with CD47 and Lymph Node
Kidney Antibodies	>12 publications with CD47 and Kidney