Dipeptidase 1 (Renal) (DPEP1) ELISA Kit for Human

Name: Human DPEP1 ELISA Kit; Dipeptidase 1 (Renal); MyBioSource
Brand: MyBioSource
SKU: MBS911972
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS911972

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DPEP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DPEP1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

dipeptidase 1 (renal) (DPEP1), ELISA Kit

Full Product Name

Human Dipeptidase 1, DPEP1 ELISA Kit

Product Gene Name

DPEP1 elisa kit

Product Synonym Names

Human Dipeptidase 1 (DPEP1) ELISA kit; MBD1; MDP; RDP; OTTHUMP00000175356; dipeptidase 1; dipeptidase 1 (renal)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates

Detection Range

23.5 pg/ml -1500 pg/ml.

Sensitivity

The minimum detectable dose of human DPEP1 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human DPEP1. No significant cross-reactivity or interference between human DPEP1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

45,674 Da

OMIM

179780

3D Structure

ModBase 3D Structure for P16444

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DPEP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DPEP1 purchase

MBS911972 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the dipeptidase 1 (renal) (DPEP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DPEP1. The ELISA analytical biochemical technique of the MBS911972 kit is based on DPEP1 antibody-DPEP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DPEP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DPEP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DPEP1. It may not necessarily be applicable to this product.

NCBI GI #

189458885

NCBI GeneID

1800

NCBI Accession #

NP_001121613.1

UniProt Primary Accession #

P16444

UniProt Secondary Accession #

Q96AK2; D3DX80

UniProt Related Accession #

P16444

GenBank Nucleotide #

NM_001128141.2

NCBI Official Full Name

dipeptidase 1

NCBI Official Synonym Full Names

dipeptidase 1 (renal)

NCBI Official Symbol

DPEP1

NCBI Official Synonym Symbols

MDP; RDP; MBD1

UniProt Protein Name

Dipeptidase 1

UniProt Synonym Protein Names

Dehydropeptidase-I; Microsomal dipeptidase; Renal dipeptidase; hRDP

Protein Family

Dipeptidase

UniProt Gene Name

DPEP1

UniProt Synonym Gene Names

MDP; RDP; hRDP

UniProt Entry Name

DPEP1_HUMAN

NCBI Protein Information

dipeptidase 1; hRDP; renal dipeptidase; dehydropeptidase-I; microsomal dipeptidase

NCBI Summary for DPEP1

The protein encoded by this gene is a kidney membrane enzyme involved in the metabolism of glutathione and other similar proteins by dipeptide hydrolysis. The encoded protein is known to regulate leukotriene activity by catalyzing the conversion of leukotriene D4 to leukotriene E4. This protein uses zinc as a cofactor and acts as a disulfide-linked homodimer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2012]

UniProt Comments for DPEP1

DPEP1: Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity. Belongs to the peptidase M19 family.

Protein type: EC 3.4.13.19; Membrane protein, GPI anchor; Protease

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: extracellular space; apical part of cell; microvillus membrane; apical plasma membrane; plasma membrane; cell junction; nucleus

Molecular Function: protein binding; metalloexopeptidase activity; zinc ion binding; dipeptidyl-peptidase activity; caspase inhibitor activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process; arachidonic acid metabolic process; leukotriene metabolic process; homocysteine metabolic process; negative regulation of caspase activity; proteolysis; negative regulation of cell migration; antibiotic metabolic process; negative regulation of apoptosis

Research Articles on DPEP1

1. Dipeptidase 1 has been identified as an excellent marker of high-grade IEN and CRC, and may thus be applied for screening of early neoplastic lesions and for prognostic stratification.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with DPEP1 elisa kit

Products by Pathway	Pathway Diagram
Eicosanoid Synthesis Pathway antibodies	Eicosanoid Synthesis Pathway Diagram
Leukotriene Biosynthesis Pathway antibodies	Leukotriene Biosynthesis Pathway Diagram
Leukotriene Biosynthesis Pathway antibodies	Leukotriene Biosynthesis Pathway Diagram

Diseases associated with DPEP1 elisa kit

Disease Name	Pubmed Publications
Carcinoma Antibodies	>5 publications with DPEP1 and Carcinoma
Cell Transformation, Neoplastic Antibodies	>2 publications with DPEP1 and Cell Transformation, Neoplastic
Kidney Neoplasms Antibodies	>1 publications with DPEP1 and Kidney Neoplasms
Adenoma Antibodies	>1 publications with DPEP1 and Adenoma
Breast Neoplasms Antibodies	>1 publications with DPEP1 and Breast Neoplasms
Kidney Diseases Antibodies	>1 publications with DPEP1 and Kidney Diseases

Organs/Tissues associated with DPEP1 elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>3 publications with DPEP1 and Kidney
Intestine Antibodies	>3 publications with DPEP1 and Intestine
Lymph Node Antibodies	>2 publications with DPEP1 and Lymph Node