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ADAM Metallopeptidase Domain 33 (ADAM33) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS922898

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM33 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM33 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ADAM metallopeptidase domain 33 (ADAM33), ELISA Kit
Full Product Name
Mouse Disintegrin and metalloproteinase domain-containing protein 33, ADAM33 ELISA Kit
Product Gene Name
ADAM33 elisa kit
Product Synonym Names
Mouse Disintegrin and metalloproteinase domain-containing protein 33 (ADAM33) ELISA kit; RP5-964F7.2; C20orf153; DJ964F7.1; DKFZp434K0521; FLJ35308; FLJ36751; MGC149823; MGC71889; a disintegrin and metalloprotease 33; a disintegrin and metalloproteinase domain 33; disintegrin and reprolys; ADAM metallopeptidase domain 33
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
15.6 pg/ml -1000 pg/ml.
Sensitivity
The minimum detectable dose of mouse ADAM33 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse ADAM33. No significant cross-reactivity or interference between mouse ADAM33 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
86,971 Da
3D Structure
ModBase 3D Structure for Q923W9
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of ADAM33 elisa kit

Mouse ADAM33 ELISA Kit – Cat# MBS922898

Other Notes

Small volumes of ADAM33 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADAM33 purchase

MBS922898 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAM metallopeptidase domain 33 (ADAM33) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAM33. The ELISA analytical biochemical technique of the MBS922898 kit is based on ADAM33 antibody-ADAM33 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM33 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM33. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ADAM33. It may not necessarily be applicable to this product.

NCBI GI #
254587924
NCBI GeneID
110751
NCBI Accession #
NP_291093.2
UniProt Primary Accession #
Q923W9
UniProt Secondary Accession #
Q8R5G5
UniProt Related Accession #
Q923W9
GenBank Nucleotide #
NM_033615.2
NCBI Official Full Name
disintegrin and metalloproteinase domain-containing protein 33 isoform 1
NCBI Official Synonym Full Names
a disintegrin and metallopeptidase domain 33
NCBI Official Symbol
Adam33
NCBI Official Synonym Symbols
Adaml
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 33
Protein Family
Disintegrin and metalloproteinase domain-containing protein
UniProt Gene Name
Adam33
UniProt Synonym Gene Names
ADAM 33
UniProt Entry Name
ADA33_MOUSE
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 33; ADAM 33; a disintegrin and metalloprotease domain 33

UniProt Comments for ADAM33

ADAM33: Genetic variations in ADAM33 are associated with susceptibility to asthma (ASTHMA). The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.4.24.-; Protease

Cellular Component: membrane; integral to membrane

Molecular Function: peptidase activity; hydrolase activity; zinc ion binding; metallopeptidase activity; metal ion binding; metalloendopeptidase activity

Biological Process: proteolysis; regulation of cytokine biosynthetic process; regulation of cell proliferation
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Research Articles on ADAM33

1. Adam33/Adam33 shows 2 significant increments in expression during lung morphogenesis, suggesting important developmental regulation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with ADAM33 elisa kit

 Disease Name  Pubmed Publications
 Chronic Disease Antibodies  >25 publications with ADAM33 and Chronic Disease
 Bronchial Hyperreactivity Antibodies  >20 publications with ADAM33 and Bronchial Hyperreactivity
 Hyperplasia Antibodies  >3 publications with ADAM33 and Hyperplasia
 ASTHMA, NASAL POLYPS, AND ASPIRIN INTOLERANCE Antibodies  >1 publications with ADAM33 and ASTHMA, NASAL POLYPS, AND ASPIRIN INTOLERANCE
 Heart Diseases Antibodies  >1 publications with ADAM33 and Heart Diseases
 Cardiovascular Diseases Antibodies  >1 publications with ADAM33 and Cardiovascular Diseases
 Myocardial Ischemia Antibodies  >1 publications with ADAM33 and Myocardial Ischemia
 Adenocarcinoma Antibodies  >1 publications with ADAM33 and Adenocarcinoma

Organs/Tissues associated with ADAM33 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >55 publications with ADAM33 and Lung
 Embryonic Tissue Antibodies  >6 publications with ADAM33 and Embryonic Tissue
 Brain Antibodies  >4 publications with ADAM33 and Brain
 Ovary Antibodies  >1 publications with ADAM33 and Ovary
 Lymph Node Antibodies  >1 publications with ADAM33 and Lymph Node
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