Fatty Acid Binding Protein 4, Adipocyte (FABP4) ELISA Kit for Mouse

Name: Mouse FABP4 ELISA Kit; Fatty Acid Binding Protein 4, Adipocyte; MyBioSource
Brand: MyBioSource
SKU: MBS925921
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS925921

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FABP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FABP4 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

fatty acid binding protein 4, adipocyte (FABP4), ELISA Kit

Full Product Name

Mouse Fatty acid-binding protein, adipocyte, FABP4 ELISA Kit

Product Gene Name

FABP4 elisa kit

Product Synonym Names

Mouse Fatty acid-binding protein; adipocyte (FABP4) ELISA kit; A-FABP; aP2; ; fatty acid binding protein 4; adipocyte

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

31.25 pg/ml -2000 pg/ml

Sensitivity

7.81 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse FABP4. No significant cross-reactivity or interference between mouse FABP4 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess

Molecular Weight

14,650 Da

3D Structure

ModBase 3D Structure for P04117

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of FABP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FABP4 purchase

MBS925921 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the fatty acid binding protein 4, adipocyte (FABP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FABP4. The ELISA analytical biochemical technique of the MBS925921 kit is based on FABP4 antibody-FABP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FABP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FABP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FABP4. It may not necessarily be applicable to this product.

NCBI GI #

14149635

NCBI GeneID

11770

NCBI Accession #

NP_077717.1

UniProt Primary Accession #

P04117

UniProt Related Accession #

P04117

GenBank Nucleotide #

NM_024406.2

NCBI Official Full Name

fatty acid-binding protein, adipocyte

NCBI Official Synonym Full Names

fatty acid binding protein 4, adipocyte

NCBI Official Symbol

Fabp4

NCBI Official Synonym Symbols

Ap2; Lbpl; 422/aP2; ALBP/Ap2

UniProt Protein Name

Fatty acid-binding protein, adipocyte

UniProt Synonym Protein Names

3T3-L1 lipid-binding protein; Adipocyte lipid-binding protein; ALBP; Adipocyte-type fatty acid-binding protein; A-FABP; AFABP; Fatty acid-binding protein 4; Myelin P2 protein homolog; P15; P2 adipocyte protein; Protein 422

Protein Family

Fatty acid-binding protein

UniProt Gene Name

Fabp4

UniProt Synonym Gene Names

Ap2; ALBP; A-FABP; AFABP

UniProt Entry Name

FABP4_MOUSE

NCBI Protein Information

fatty acid-binding protein, adipocyte; P15; ALBP; AFABP; A-FABP; protein 422; P2 adipocyte protein; adipocyte protein aP2; myelin P2 protein homolog; 3T3-L1 lipid-binding protein; fatty acid-binding protein 4; adipocyte lipid-binding protein; adipocyte-type fatty acid-binding protein

UniProt Comments for FABP4

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: transporter activity; lipid binding

Biological Process: cholesterol homeostasis; transport; positive regulation of cell proliferation; white fat cell differentiation; brown fat cell differentiation; cytokine production; negative regulation of protein kinase activity; fatty acid metabolic process; negative regulation of transcription, DNA-dependent; positive regulation of inflammatory response

Research Articles on FABP4

1. found that the FABP4 level was higher and PPARgamma level was lower in human visceral fat and mouse epididymal fat compared with their subcutaneous fat

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FABP4 elisa kit

Reference Product	PubMed Publications
Pparg elisa kit	>103 publications with FABP4 and Pparg
Cebpa elisa kit	>26 publications with FABP4 and Cebpa
Cebpb elisa kit	>4 publications with FABP4 and Cebpb
Sirt1 elisa kit	>1 publications with FABP4 and Sirt1

Pathways associated with FABP4 elisa kit

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway antibodies	Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway Diagram
Lipid Digestion, Mobilization, And Transport Pathway antibodies	Lipid Digestion, Mobilization, And Transport Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway antibodies	PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway Diagram
PPAR Signaling Pathway antibodies	PPAR Signaling Pathway Diagram
PPAR Signaling Pathway antibodies	PPAR Signaling Pathway Diagram
Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies	Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram

Diseases associated with FABP4 elisa kit

Disease Name	Pubmed Publications
Obesity Antibodies	>108 publications with FABP4 and Obesity
Insulin Resistance Antibodies	>80 publications with FABP4 and Insulin Resistance
Inflammation Antibodies	>51 publications with FABP4 and Inflammation
Hyperinsulinism Antibodies	>48 publications with FABP4 and Hyperinsulinism
Atherosclerosis Antibodies	>47 publications with FABP4 and Atherosclerosis
Cardiovascular Diseases Antibodies	>40 publications with FABP4 and Cardiovascular Diseases
Diabetes Mellitus Antibodies	>35 publications with FABP4 and Diabetes Mellitus
Fatty Liver Antibodies	>32 publications with FABP4 and Fatty Liver
Necrosis Antibodies	>20 publications with FABP4 and Necrosis
Neoplasms Antibodies	>18 publications with FABP4 and Neoplasms

Organs/Tissues associated with FABP4 elisa kit

Organ/Tissue Name	Pubmed Publications
Adipose Tissue Antibodies	>134 publications with FABP4 and Adipose Tissue
Connective Tissue Antibodies	>88 publications with FABP4 and Connective Tissue
Muscle Antibodies	>53 publications with FABP4 and Muscle
Liver Antibodies	>39 publications with FABP4 and Liver
Bone Marrow Antibodies	>25 publications with FABP4 and Bone Marrow
Heart Antibodies	>19 publications with FABP4 and Heart
Embryonic Tissue Antibodies	>11 publications with FABP4 and Embryonic Tissue
Kidney Antibodies	>8 publications with FABP4 and Kidney
Lung Antibodies	>8 publications with FABP4 and Lung
Eye Antibodies	>6 publications with FABP4 and Eye