Apolipoprotein C-III (APOC3) ELISA Kit for Rat

Name: Rat APOC3 ELISA Kit; Apolipoprotein C-III; MyBioSource
Brand: MyBioSource
SKU: MBS929891
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS929891

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with APOC3. Standards or samples are added to the appropriate microtiter plate wells with… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

apolipoprotein C-III (APOC3), ELISA Kit

Full Product Name

Rat Apolipoprotein C-III, APOC3 ELISA Kit

Product Gene Name

APOC3 elisa kit

Product Synonym Names

Rat Apolipoprotein C-III (APOC3) ELISA kit; APOCIII; MGC150353; ; apolipoprotein C-III

Species Reactivity

Rat

Assay Type

Competitive

Samples

Serum, plasma, tissue homogenates.

Detection Range

31.25 ng/ml-2000 ng/ml.

Sensitivity

The minimum detectable dose of rat APOC3 is typically less than 7.8 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of rat APOC3. No significant cross-reactivity or interference between rat APOC3 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

11,117 Da

3D Structure

ModBase 3D Structure for P06759

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of APOC3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APOC3 purchase

MBS929891 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apolipoprotein C-III (APOC3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOC3. The ELISA analytical biochemical technique of the MBS929891 kit is based on APOC3 antibody-APOC3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOC3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOC3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for APOC3. It may not necessarily be applicable to this product.

NCBI GI #

6226553

NCBI GeneID

24207

NCBI Accession #

P06759.2

UniProt Primary Accession #

P06759

UniProt Related Accession #

P06759

NCBI Official Full Name

Apolipoprotein C-III

NCBI Official Synonym Full Names

apolipoprotein C-III

NCBI Official Symbol

Apoc3

NCBI Official Synonym Symbols

ApoC-III; apo-CIII

UniProt Protein Name

Apolipoprotein C-III

UniProt Synonym Protein Names

Apolipoprotein C3

Protein Family

Apolipoprotein

UniProt Gene Name

Apoc3

UniProt Synonym Gene Names

Apo-CIII; ApoC-III

UniProt Entry Name

APOC3_RAT

NCBI Protein Information

apolipoprotein C-III; apolipoprotein C3; apolipoprotein C-3

NCBI Summary for APOC3

very low density lipoprotein (VLDL) that comprises a major component of the lipid transport system; increased levels induce hypertriglyceridemia [RGD, Feb 2006]

UniProt Comments for APOC3

APOC3: Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors

Protein type: Lipid-binding; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; chylomicron

Molecular Function: lipid transporter activity; lipase inhibitor activity; phospholipid binding; enzyme regulator activity

Biological Process: response to drug; cholesterol metabolic process; response to peptide hormone stimulus; regulation of Cdc42 protein signal transduction; cholesterol efflux; lipoprotein metabolic process; lipid transport; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; triacylglycerol metabolic process; negative regulation of lipid catabolic process; phospholipid efflux; lipoprotein transport; triacylglycerol catabolic process; negative regulation of receptor-mediated endocytosis; negative regulation of lipoprotein lipase activity; negative regulation of lipid metabolic process; triacylglycerol mobilization; inflammatory response; response to nutrient; negative regulation of fatty acid biosynthetic process

Research Articles on APOC3

1. ApoCIII forms a abundant ribbonlike loop of amyloidogenic protein in triangles and squares with a width of 50 nm.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with APOC3 elisa kit

Products by Pathway	Pathway Diagram
PPAR Signaling Pathway antibodies	PPAR Signaling Pathway Diagram
PPAR Signaling Pathway antibodies	PPAR Signaling Pathway Diagram
Statin Pathway antibodies	Statin Pathway Diagram

Diseases associated with APOC3 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>67 publications with APOC3 and Cardiovascular Diseases
Coronary Artery Disease Antibodies	>47 publications with APOC3 and Coronary Artery Disease
Coronary Disease Antibodies	>47 publications with APOC3 and Coronary Disease
Myocardial Ischemia Antibodies	>45 publications with APOC3 and Myocardial Ischemia
Dyslipidemias Antibodies	>41 publications with APOC3 and Dyslipidemias
Hypertriglyceridemia Antibodies	>41 publications with APOC3 and Hypertriglyceridemia
Heart Diseases Antibodies	>41 publications with APOC3 and Heart Diseases
Hyperlipidemias Antibodies	>34 publications with APOC3 and Hyperlipidemias
Atherosclerosis Antibodies	>31 publications with APOC3 and Atherosclerosis
Insulin Resistance Antibodies	>30 publications with APOC3 and Insulin Resistance