Human HIST1H3A ELISA Kit – Cat# MBS937951

Histone Cluster 1, H3a (HIST1H3A) ELISA Kit for Human

Name: Human HIST1H3A ELISA Kit; Histone Cluster 1, H3a; MyBioSource
Brand: MyBioSource
SKU: MBS937951
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS937951

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIST1H3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIST1H3A present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

histone cluster 1, H3a (HIST1H3A), ELISA Kit

Full Product Name

Human Histone H3.1, HIST1H3A ELISA Kit

Product Gene Name

HIST1H3A elisa kit

Product Synonym Names

Human Histone H3.1 (HIST1H3A) ELISA kit; H3/A; H3FA; H3 histone family; member A; histone 1; H3a; histone cluster 1; H3a

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates

Detection Range

23.5 pg/ml-1500 pg/ml

Sensitivity

< 5.8 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human HIST1H3A. No significant cross-reactivity or interference between human HIST1H3A and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

15,404 Da

OMIM

gene 602819

3D Structure

ModBase 3D Structure for P68431

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of HIST1H3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIST1H3A purchase

MBS937951 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the histone cluster 1, H3a (HIST1H3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HIST1H3A. The ELISA analytical biochemical technique of the MBS937951 kit is based on HIST1H3A antibody-HIST1H3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIST1H3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIST1H3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HIST1H3A. It may not necessarily be applicable to this product.

NCBI GI #

4504281

NCBI GeneID

8350

NCBI Accession #

NP_003520.1

UniProt Primary Accession #

P68431

UniProt Secondary Accession #

P02295; P02296; P16106; Q6ISV8; Q6NWP8; Q6NWP9; Q6NXU4; Q71DJ3; Q93081; A0PJT7; A5PLR1

UniProt Related Accession #

P68431

GenBank Nucleotide #

NM_003529.2

NCBI Official Full Name

histone H3.1

NCBI Official Synonym Full Names

histone cluster 1, H3a

NCBI Official Symbol

HIST1H3A

NCBI Official Synonym Symbols

H3/A; H3FA

UniProt Protein Name

Histone H3.1

UniProt Synonym Protein Names

Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l

UniProt Gene Name

HIST1H3A

UniProt Synonym Gene Names

H3FA

UniProt Entry Name

H31_HUMAN

NCBI Protein Information

histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A

NCBI Summary for HIST1H3A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

UniProt Comments for HIST1H3A

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; DNA replication-dependent nucleosome assembly; DNA methylation on cytosine; regulation of gene expression, epigenetic

Research Articles on HIST1H3A

1. The authors report that de novo CENP-A assembly and kinetochore formation on human centromeric alphoid DNA arrays is regulated by a histone H3K9 acetyl/methyl balance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with HIST1H3A elisa kit

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Organs/Tissues associated with HIST1H3A elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with HIST1H3A and Brain
Blood Antibodies	>1 publications with HIST1H3A and Blood