Lysine (K) -Specific Demethylase 5a (KDM5A) ELISA Kit for Human

Name: Human KDM5A ELISA Kit; Lysine (K) -Specific Demethylase 5a; MyBioSource
Brand: MyBioSource
SKU: MBS938758
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS938758

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KDM5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KDM5A present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

lysine (K) -specific demethylase 5A (KDM5A), ELISA Kit

Full Product Name

Human Lysine-specific demethylase 5A, KDM5A ELISA Kit

Product Gene Name

KDM5A elisa kit

Product Synonym Names

Human Lysine-specific demethylase 5A (KDM5A) ELISA kit; JARID1A; RBBP2; RBP2; Jumonji; AT rich interactive domain 1A (RBBP2-like) ; Jumonji; AT rich interactive domain 1A (RBP2-like) ; retinoblastoma binding protein 2; retinoblastoma-binding protein 2; lysine (K) -specific demethylase 5A

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates

Detection Range

23.5 pg/ml -1500 pg/ml.

Sensitivity

The minimum detectable dose of human KDM5A is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human KDM5A. No significant cross-reactivity or interference between human KDM5A and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

192,095 Da

OMIM

180202

3D Structure

ModBase 3D Structure for P29375

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of KDM5A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KDM5A purchase

MBS938758 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lysine (K) -specific demethylase 5A (KDM5A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KDM5A. The ELISA analytical biochemical technique of the MBS938758 kit is based on KDM5A antibody-KDM5A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KDM5A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KDM5A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for KDM5A. It may not necessarily be applicable to this product.

NCBI GI #

110618244

NCBI GeneID

5927

NCBI Accession #

NP_001036068.1

UniProt Primary Accession #

P29375

UniProt Secondary Accession #

Q4LE72; Q86XZ1; A8MV76

UniProt Related Accession #

P29375

GenBank Nucleotide #

NM_001042603.2

NCBI Official Full Name

lysine-specific demethylase 5A

NCBI Official Synonym Full Names

lysine (K)-specific demethylase 5A

NCBI Official Symbol

KDM5A

NCBI Official Synonym Symbols

RBP2; RBBP2; RBBP-2

UniProt Protein Name

Lysine-specific demethylase 5A

UniProt Synonym Protein Names

Histone demethylase JARID1A; Jumonji/ARID domain-containing protein 1A; Retinoblastoma-binding protein 2; RBBP-2

Protein Family

Lysine-specific demethylase

UniProt Gene Name

KDM5A

UniProt Synonym Gene Names

JARID1A; RBBP2; RBP2; RBBP-2

UniProt Entry Name

KDM5A_HUMAN

NCBI Protein Information

lysine-specific demethylase 5A; histone demethylase JARID1A; retinoblastoma binding protein 2; jumonji/ARID domain-containing protein 1A; Jumonji, AT rich interactive domain 1A (RBP2-like)

NCBI Summary for KDM5A

This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013]

UniProt Comments for KDM5A

JARID1A: Histone demethylase that specifically demethylates 'Lys- 4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Interacts with SUZ12; the interaction is direct. Interacts with the viral protein-binding domain of RB1. Interacts with ESR1, MYC, MYCN and LMO2. Belongs to the JARID1 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 1.14.11.-; Nucleolus; Oxidoreductase; Transcription factor

Chromosomal Location of Human Ortholog: 12p11

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: protein binding; DNA binding; zinc ion binding; transcription coactivator activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; multicellular organismal development; positive regulation of transcription, DNA-dependent; chromatin modification; circadian regulation of gene expression

Research Articles on KDM5A

1. JARID1A, JMY, and PTGER4 polymorphisms are related to ankylosing spondylitis in Chinese Han patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KDM5A elisa kit

Reference Product	PubMed Publications
RB1 elisa kit	>3 publications with KDM5A and RB1
TBP elisa kit	>1 publications with KDM5A and TBP
RBL1 elisa kit	>1 publications with KDM5A and RBL1
LMO2 elisa kit	>1 publications with KDM5A and LMO2

Organs/Tissues associated with KDM5A elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3 publications with KDM5A and Blood
Nerve Antibodies	>3 publications with KDM5A and Nerve
Lung Antibodies	>3 publications with KDM5A and Lung
Skin Antibodies	>2 publications with KDM5A and Skin
Muscle Antibodies	>2 publications with KDM5A and Muscle
Bladder Antibodies	>2 publications with KDM5A and Bladder
Embryonic Tissue Antibodies	>1 publications with KDM5A and Embryonic Tissue
Prostate Antibodies	>1 publications with KDM5A and Prostate
Placenta Antibodies	>1 publications with KDM5A and Placenta
Bone Antibodies	>1 publications with KDM5A and Bone