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RAB10, Member RAS Oncogene Family (RAB10) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS939722

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RAB10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAB10 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RAB10, member RAS oncogene family, ELISA Kit
Full Product Name
Human Ras-related protein Rab-10, RAB10 ELISA Kit
Product Gene Name
RAB10 elisa kit
Product Synonym Names
Human Ras-related protein Rab-10 (RAB10) ELISA kit; GTP-binding protein RAB10; ras-related GTP-binding protein; ras-related GTP-binding protein RAB10; RAB10; member RAS oncogene family
Species Reactivity
Human
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates, Cell lysates
Detection Range
39 pg/ml -2500 pg/ml.
Sensitivity
The minimum detectable dose of human RAB10 is typically less than 9.77 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Specificity
This assay has high sensitivity and excellent specificity for detection of human RAB10. No significant cross-reactivity or interference between human RAB10 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
22,541 Da
OMIM
612672
3D Structure
ModBase 3D Structure for P61026
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of RAB10 elisa kit

Human RAB10 ELISA Kit – Cat# MBS939722

Other Notes

Small volumes of RAB10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAB10 purchase

MBS939722 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RAB10, member RAS oncogene family, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RAB10. The ELISA analytical biochemical technique of the MBS939722 kit is based on RAB10 antibody-RAB10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RAB10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RAB10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for RAB10. It may not necessarily be applicable to this product.

NCBI GI #
256222019
NCBI GeneID
10890
NCBI Accession #
NP_057215.3
UniProt Primary Accession #
P61026
UniProt Secondary Accession #
O88386; Q6IA52; Q9D7X6; Q9H0T3; D6W538
UniProt Related Accession #
P61026
GenBank Nucleotide #
NM_016131.4
NCBI Official Full Name
ras-related protein Rab-10
NCBI Official Synonym Full Names
RAB10, member RAS oncogene family
NCBI Official Symbol
RAB10
UniProt Protein Name
Ras-related protein Rab-10
Protein Family
Ras-related protein
UniProt Gene Name
RAB10
UniProt Entry Name
RAB10_HUMAN
NCBI Protein Information
ras-related protein Rab-10; GTP-binding protein RAB10; ras-related GTP-binding protein

NCBI Summary for RAB10

RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking (Bao et al., 1998 [PubMed 9918381]).[supplied by OMIM, Mar 2009]

UniProt Comments for RAB10

RAB10: a protein of the GTPase superfamily, Rab family. May be involved in vesicular trafficking and neurotransmitter release. Localized to the inner face of the cell membrane by a lipid-anchor.

Protein type: G protein, monomeric, Rab; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: Golgi apparatus; recycling endosome; endoplasmic reticulum membrane; phagocytic vesicle membrane; focal adhesion; cytoplasmic vesicle membrane; recycling endosome membrane; exocyst; plasma membrane; endosome membrane; trans-Golgi network; endosome

Molecular Function: GTPase activity; GDP binding; GTP binding; myosin V binding

Biological Process: metabolic process; establishment of neuroblast polarity; protein secretion; endosome transport; vesicle-mediated transport; regulation of exocytosis; intracellular protein transport; polarized epithelial cell differentiation; Golgi to plasma membrane transport; antigen processing and presentation; axonogenesis; cellular response to insulin stimulus; Golgi to plasma membrane protein transport; Rab protein signal transduction; vesicle docking during exocytosis
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Research Articles on RAB10

1. Rab10 silencing increased the plasma membrane residence of HAS3, resulting in a significant increase of hyaluronan secretion and an enlarged cell surface HA coat, whereas Rab10 overexpression suppressed hyaluronan synthesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with RAB10 elisa kit

 Reference Product  PubMed Publications
 RIMS1 elisa kit  >1 publications with RAB10 and RIMS1

Diseases associated with RAB10 elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with RAB10 and Neoplasms
 Genital Diseases, Male Antibodies  >1 publications with RAB10 and Genital Diseases, Male
 Gonadal Disorders Antibodies  >1 publications with RAB10 and Gonadal Disorders
 Carcinoma Antibodies  >1 publications with RAB10 and Carcinoma
 Carcinoma, Hepatocellular Antibodies  >1 publications with RAB10 and Carcinoma, Hepatocellular
 Disease Models, Animal Antibodies  >1 publications with RAB10 and Disease Models, Animal
 Edema Antibodies  >1 publications with RAB10 and Edema
 Liver Neoplasms Antibodies  >1 publications with RAB10 and Liver Neoplasms
 Weight Loss Antibodies  >1 publications with RAB10 and Weight Loss
 Liver Diseases Antibodies  >1 publications with RAB10 and Liver Diseases

Organs/Tissues associated with RAB10 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >9 publications with RAB10 and Muscle
 Brain Antibodies  >7 publications with RAB10 and Brain
 Kidney Antibodies  >5 publications with RAB10 and Kidney
 Liver Antibodies  >2 publications with RAB10 and Liver
 Placenta Antibodies  >2 publications with RAB10 and Placenta
 Lung Antibodies  >2 publications with RAB10 and Lung
 Intestine Antibodies  >2 publications with RAB10 and Intestine
 Connective Tissue Antibodies  >1 publications with RAB10 and Connective Tissue
 Pancreas Antibodies  >1 publications with RAB10 and Pancreas
 Vascular Antibodies  >1 publications with RAB10 and Vascular
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