Wingless-Type MMTV Integration Site Family, Member 5b (WNT5B) ELISA Kit for Human

Name: Human WNT5B ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS941658
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS941658

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT5B present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

wingless-type MMTV integration site family, member 5B (WNT5B), ELISA Kit

Full Product Name

Human Protein Wnt-5b, WNT5B ELISA Kit

Product Gene Name

WNT5B elisa kit

Product Synonym Names

Human Protein Wnt-5b (WNT5B) ELISA kit; MGC2648; WNT-5B protein; wingless-type MMTV integration site family; member 5B

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.156 ng/ml-10 ng/ml

Sensitivity

< 0.039 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human WNT5B. No significant cross-reactivity or interference between human WNT5B and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

40,323 Da

OMIM

606361

3D Structure

ModBase 3D Structure for Q9H1J7

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of WNT5B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WNT5B purchase

MBS941658 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the wingless-type MMTV integration site family, member 5B (WNT5B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT5B. The ELISA analytical biochemical technique of the MBS941658 kit is based on WNT5B antibody-WNT5B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT5B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT5B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for WNT5B. It may not necessarily be applicable to this product.

NCBI GI #

17402919

NCBI GeneID

81029

NCBI Accession #

NP_110402.2

UniProt Primary Accession #

Q9H1J7

UniProt Secondary Accession #

Q96S49; Q9BV04; A8K315; D3DUP9

UniProt Related Accession #

Q9H1J7

GenBank Nucleotide #

NM_030775.2

NCBI Official Full Name

protein Wnt-5b

NCBI Official Synonym Full Names

wingless-type MMTV integration site family, member 5B

NCBI Official Symbol

WNT5B

UniProt Protein Name

Protein Wnt-5b

UniProt Gene Name

WNT5B

UniProt Entry Name

WNT5B_HUMAN

NCBI Protein Information

protein Wnt-5b; WNT-5B protein

NCBI Summary for WNT5B

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]

UniProt Comments for WNT5B

WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; plasma membrane; extracellular region

Molecular Function: frizzled binding; receptor binding

Biological Process: fat cell differentiation; neuron differentiation; cell fate commitment; Wnt receptor signaling pathway; wound healing; chondrocyte differentiation; positive regulation of fat cell differentiation; positive regulation of cell migration

Research Articles on WNT5B

1. Ursolic acid suppressed the expression of Wnt5alpha/beta and beta-catenin, inducing apoptosis in prostate cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with WNT5B elisa kit

Reference Product	PubMed Publications
PORCN elisa kit	>1 publications with WNT5B and PORCN

Pathways associated with WNT5B elisa kit

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Basal Cell Carcinoma Pathway antibodies	Basal Cell Carcinoma Pathway Diagram
Basal Cell Carcinoma Pathway antibodies	Basal Cell Carcinoma Pathway Diagram
DNA Damage Response (only ATM Dependent) Pathway antibodies	DNA Damage Response (only ATM Dependent) Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
Hedgehog Signaling Pathway antibodies	Hedgehog Signaling Pathway Diagram
Hedgehog Signaling Pathway antibodies	Hedgehog Signaling Pathway Diagram
Melanogenesis Pathway antibodies	Melanogenesis Pathway Diagram
Melanogenesis Pathway antibodies	Melanogenesis Pathway Diagram

Diseases associated with WNT5B elisa kit

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>8 publications with WNT5B and Breast Neoplasms
Skin Diseases Antibodies	>6 publications with WNT5B and Skin Diseases
Stomach Diseases Antibodies	>4 publications with WNT5B and Stomach Diseases
Disease Models, Animal Antibodies	>4 publications with WNT5B and Disease Models, Animal
Fibrosis Antibodies	>3 publications with WNT5B and Fibrosis
Drug Toxicity Antibodies	>3 publications with WNT5B and Drug Toxicity
Necrosis Antibodies	>3 publications with WNT5B and Necrosis
Brain Diseases Antibodies	>3 publications with WNT5B and Brain Diseases
Heart Defects, Congenital Antibodies	>2 publications with WNT5B and Heart Defects, Congenital
Liver Diseases Antibodies	>2 publications with WNT5B and Liver Diseases

Organs/Tissues associated with WNT5B elisa kit

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>18 publications with WNT5B and Embryonic Tissue
Bone Antibodies	>15 publications with WNT5B and Bone
Muscle Antibodies	>12 publications with WNT5B and Muscle
Brain Antibodies	>9 publications with WNT5B and Brain
Lung Antibodies	>8 publications with WNT5B and Lung
Kidney Antibodies	>8 publications with WNT5B and Kidney
Eye Antibodies	>7 publications with WNT5B and Eye
Liver Antibodies	>6 publications with WNT5B and Liver
Heart Antibodies	>6 publications with WNT5B and Heart
Intestine Antibodies	>5 publications with WNT5B and Intestine