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Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS947608

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNFSF13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFSF13 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
tumor necrosis factor (ligand) superfamily, member 13 (TNFSF13), ELISA Kit
Full Product Name
Mouse Tumor necrosis factor ligand superfamily member 13, TNFSF13 ELISA Kit
Product Gene Name
Product Synonym Names
Mouse Tumor necrosis factor ligand superfamily member 13 (TNFSF13) ELISA kit; APRIL; CD256; TALL2; TRDL-1; UNQ383/PRO715; ligand; TNF- and APOL-related leukocyte expressed ligand 2; a proliferation inducing ligand; tumor necrosis factor (ligand) superfamily member 13 transcript; tumor necrosis factor (ligand) superfamily; member 13
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates
Detection Range
93.75 pg/ml -6000 pg/ml.
Sensitivity
The minimum detectable dose of mouse TNFSF13 is typically less than 23.43 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse TNFSF13. No significant cross-reactivity or interference between mouse TNFSF13 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
26,889 Da
3D Structure
ModBase 3D Structure for Q9D777
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of TNFSF13 elisa kit

Mouse TNFSF13 ELISA Kit – Cat# MBS947608

Other Notes

Small volumes of TNFSF13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNFSF13 purchase

MBS947608 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tumor necrosis factor (ligand) superfamily, member 13 (TNFSF13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNFSF13. The ELISA analytical biochemical technique of the MBS947608 kit is based on TNFSF13 antibody-TNFSF13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNFSF13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNFSF13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TNFSF13. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 13 isoform 2
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 13
NCBI Official Symbol
Tnfsf13
NCBI Official Synonym Symbols
April; Tall2; Trdl1; 2310026N09Rik
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 13
UniProt Synonym Protein Names
A proliferation-inducing ligand; APRIL; CD_antigen: CD256
UniProt Gene Name
Tnfsf13
UniProt Synonym Gene Names
April; APRIL
UniProt Entry Name
TNF13_MOUSE
NCBI Protein Information
tumor necrosis factor ligand superfamily member 13; a proliferation-inducing ligand

UniProt Comments for TNFSF13

TNFSF13: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Belongs to the tumor necrosis factor family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Cellular Component: extracellular space; membrane; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor binding

Biological Process: regulation of immune response; immune system process; immunoglobulin production in mucosal tissue; positive regulation of cell proliferation; positive regulation of germinal center formation; immune response; immunoglobulin mediated immune response; positive regulation of isotype switching to IgA isotypes
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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