Polyadenylate-Binding Protein 1 (PABPC1) Human Recombinant Protein

Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing prature stop codons; for the recognition of prature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed.

Human mRNA polyadenylate binding protein evolutionary conservation of a nucleic acid binding motif.Grange T., de Sa C.M., Oddos J., Pictet R.Nucleic Acids Res. 15:4771-4787(1987) The human poly(A) -binding protein (PABP) gene structural and functional analysis.Hornstein E., Abramzon-Talianker A., Wiesel I., Meyuhas O. DNA sequence and analysis of human chromosome 8.Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.Nature 439:331-335(2006) Nucleotide sequence of a partial cDNA encoding a novel human polyadenylate-binding protein.Murphy E.P., McKenna N.J., Headon D.R.Bienvenut W.V., Zebisch A., Lilla S., von Kriegsheim A., Lempens A., Kolch W.Submitted (DEC-2008) to UniProtKB Bienvenut W.V., Waridel P., Quadroni M.Submitted (MAR-2009) to UniProtKB The mRNA poly(A) -binding protein localization, abundance, and RNA-binding specificity.Goerlach M., Burd C.G., Dreyfuss G.Exp. Cell Res. 211:400-407(1994) The human poly(A) -binding protein 1 shuttles between the nucleus and the cytoplasm.Afonina E., Stauber R., Pavlakis G.N.J. Biol. Chem. 273:13015-13021(1998) Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation.Craig A.W.B., Haghighat A., Yu A.T.K., Sonenberg N.Nature 392:520-523(1998) A mechanism for translationally coupled mRNA turnover interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex.Grosset C., Chen C.-Y.A., Xu N., Sonenberg N., Jacquemin-Sablon H., Shyu A.-B.Cell 103:29-40(2000) Translational repression by a novel partner of human poly(A) binding protein, Paip2.Khaleghpour K., Svitkin Y.V., Craig A.W.B., De;Maria C.T., Deo R.C., Burley S.K., Sonenberg N.Mol. Cell 7:205-216(2001) Dual interactions of the translational repressor Paip2 with poly(A) binding protein.Khaleghpour K., Kahvejian A., De Crescenzo G., Roy G., Svitkin Y.V., Imataka H., O'Connor-McCourt M., Sonenberg N.Mol. Cell. Biol. 21:5200-5213(2001) PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays.Lee J., Bedford M.T.EMBO Rep. 3:268-273(2002) Paip1 interacts with poly(A) binding protein through two independent binding motifs.Roy G., De Crescenzo G., Khaleghpour K., Kahvejian A., O'Connor-McCourt M., Sonenberg N.Mol. Cell. Biol. 22:3769-3782(2002) Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.RNA 8:426-439(2002) Affinity purification of ARE-binding proteins identifies polyA-binding protein 1 as a potential substrate in MK2-induced mRNA stabilization.Bollig F., Winzen R., Gaestel M., Kostka S., Resch K., Holtmann H.Biochem. Biophys. Res. Commun. 301:665-670(2003) UNR, a new partner of poly(A) -binding protein, plays a key role in translationally coupled mRNA turnover mediated by the c-fos major coding-region determinant.Chang T.-C., Yamashita A., Chen C.-Y.A., Yamashita Y., Zhu W., Durdan S., Kahvejian A., Sonenberg N., Shyu A.-B.Genes Dev. 18:2010-2023(2004) The autoregulatory translational control element of poly(A) -binding protein mRNA forms a heteromeric ribonucleoprotein complex.Patel G.P., Ma S., Bag J.Nucleic Acids Res. 33:7074-7089(2005) IMP1 interacts with poly(A) -binding protein (PABP) and the autoregulatory translational control element of PABP-mRNA through the KH III-IV domain.Patel G.P., Bag J.FEBS J. 273:5678-5690(2006) Regulation of poly(A) binding protein function in translation Characterization of the Paip2 homolog, Paip2B.Berlanga J.J., Baass A., Sonenberg N.RNA 12:1556-1568(2006) Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.Hoeck J., Weinmann L., Ender C., Ruedel S., Kremmer E., Raabe M., Urlaub H., Meister G.EMBO Rep. 8:1052-1060(2007) NFX1-123 and poly(A) binding proteins synergistically augment activation of telomerase in human papillomavirus type 16 E6-expressing cells.Katzenellenbogen R.A., Egelkrout E.M., Vliet-Gregg P., Gewin L.C., Gafken P.R., Galloway D.A.J. 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U.S.A. 105:10762-10767(2008) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.Anal. Chem. 81:4493-4501(2009) Control of c-myc mRNA stability by IGF2BP1-associated cytoplasmic RNPs.Weidensdorfer D., Stoehr N., Baude A., Lederer M., Koehn M., Schierhorn A., Buchmeier S., Wahle E., Huettelmaiery S.RNA 15:104-115(2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.Science 325:834-840(2009) Poly(A) -binding protein (PABP) a common viral target.Smith R.W., Gray N.K.Biochem. J. 426:1-12(2010) The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration.Burrows C., Abd Latip N., Lam S.J., Carpenter L., Sawicka K., Tzolovsky G., Gabra H., Bushell M., Glover D.M., Willis A.E., Blagden S.P.Nucleic Acids Res. 38:5542-5553(2010) Human cytomegalovirus UL69 protein facilitates translation by associating with the mRNA cap-binding complex and excluding 4EBP1.Aoyagi M., Gaspar M., Shenk T.E.Proc. Natl. Acad. Sci. U.S.A. 107:2640-2645(2010) A stimulatory role for the La-related protein 4B in translation.Schaffler K., Schulz K., Hirmer A., Wiesner J., Grimm M., Sickmann A., Fischer U.RNA 16:1488-1499(2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.Sci. Signal. 3:RA3-RA3(2010) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) Human senataxin resolves RNA/DNA hybrids formed at transcriptional pause sites to promote Xrn2-dependent termination.Skourti-Stathaki K., Proudfoot N.J., Gromak N.Mol. Cell 42:794-805(2011) Proteomic analysis of cap-dependent translation identifies LARP1 as a key regulator of 5'TOP mRNA translation.Tcherkezian J., Cargnello M., Romeo Y., Huttlin E.L., Lavoie G., Gygi S.P., Roux P.P.Genes Dev. 28:357-371(2014) Recognition of polyadenylate RNA by the poly(A) -binding protein.Deo R.C., Bonanno J.B., Sonenberg N., Burley S.K.Cell 98:835-845(1999) Structure and function of the C-terminal PABC domain of human poly(A) -binding protein.Kozlov G., Trempe J.F., Khaleghpour K., Kahvejian A., Ekiel I., Gehring K.Proc. Natl. Acad. Sci. U.S.A. 98:4409-4413(2001) Eukaryotic translation termination factor Gspt/ERF3 recognizes Pabp with chemical exchange using two overlapping motifs.Osawa M., Nakanishi T., Hosoda N., Uchida S., Hoshino T., Katada I., Shimada I.Submitted (MAY-2009) to the PDB data bankMolecular determinants of PAM2 recognition by the MLLE domain of poly(A) -binding protein.Kozlov G., Menade M., Rosenauer A., Nguyen L., Gehring K.J. Mol. Biol. 397:397-407(2010) +Additional computationally mapped references.<p>Provides general information on the entry.

Small volumes of PABPC1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS960232 is a recombinant protein targeting PABPC1. Also known as Recombinant Human Polyadenylate-binding protein 1. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.