Mouse CTCFL Recombinant Protein – Cat# MBS967458

Transcriptional Repressor CTCFL (CTCFL) Mouse Recombinant Protein

Name: CTCFL Recombinant Protein; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS967458
Price: 315 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS967458

Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating… More details

$315 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Transcriptional repressor CTCFL, Recombinant Protein

Full Product Name

Recombinant Mouse Transcriptional repressor CTCFL

Product Gene Name

Ctcfl recombinant protein

Product Synonym Names

Brother of the regulator of imprinted sites; CCCTC-binding factor; CTCF paralog; CTCF-like protein

Sequence

MAAAEVPVPS GYFTQIKEQK LKPGDLEEEK EEDGVQRVEA QEGVVKEVEA ENSCLLLEAR APVESDRRIL TLQTVHLESQ DVHLQGLGWL SVPHSEELSG TVPEAEGILQ LPSVLWLDPE PQLSLQHCVT VSIPEELYPP EELQRIHFHL LRENVLMAEE NPELTPDLDE STALKKPEED EKDQLPPQGE TDKREERLLL LEMKPKEGKD DEIVLTISHL SLEEQQDPPA ANQTSVPGAK AAKPKRRRQT KGKPQSFQCD TCPFTSSKLS TFNRHIKIHS NERPHLCHLC LKAFRTVTLL RNHVNTHTGT RPHKCRDCDM AFVTSGELVR HRRYKHTYEK PFKCSLCKYA SVEASKMKRH IRSHTGERPF QCCQCAYASR DSYKLKRHMR THSGEKPYEC PTCHVRFTQS GTMKIHIAQK HGENVPKYEC PHCATIIARK SDLRVHLRNL HSQSPEEMKC RYCPAGFHER YALIQHQRTH KNEKKFKCKQ CDYACKQERC LKAHMRMHTG EKPFSCLACN KHFRQKQLLT VHLRKYHDPN FVPNLHLCLK CDKRFSRWSN LQRHRKKCDP EHETLAPNKD RRPVTRTQAS EGEAGHKEGE PQCPGEQALG HQGEAAGSQS PDHGLTCEII FNMMDK

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Molecular Weight

75.1 kDa

Sequence Positions

1-636. Full Length

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

3D Structure

ModBase 3D Structure for A2APF3

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for Ctcfl recombinant protein

BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma.Loukinov D.I., Pugacheva E., Vatolin S., Pack S.D., Moon H., Chernukhin I., Mannan P., Larsson E., Kanduri C., Vostrov A.A., Cui H., Niemitz E.L., Rasko J.E.J., Docquier F.M., Kistler M., Breen J.J., Zhuang Z., Quitschke W.W., Renkawitz R., Klenova E.M., Feinberg A.P., Ohlsson R., Morse H.C. III, Lobanenkov V.V.Proc. Natl. Acad. Sci. U.S.A. 99:6806-6811(2002) Lineage-specific biology revealed by a finished genome assembly of the mouse.Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) The testis-specific factor CTCFL cooperates with the protein methyltransferase PRMT7 in H19 imprinting control region methylation.Jelinic P., Stehle J.-C., Shaw P.PLoS Biol. 4:E355-E355(2006)

Other Notes

Small volumes of Ctcfl recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ctcfl purchase

MBS967458 is a recombinant protein targeting Ctcfl. Also known as Recombinant Mouse Transcriptional repressor CTCFL. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for Ctcfl. It may not necessarily be applicable to this product.

NCBI GI #

124487069

NCBI GeneID

664799

NCBI Accession #

NP_001074856.1

UniProt Primary Accession #

A2APF3

UniProt Secondary Accession #

Q3Y6S0; B9EKP6

UniProt Related Accession #

A2APF3

GenBank Nucleotide #

NM_001081387.2

NCBI Official Full Name

transcriptional repressor CTCFL

NCBI Official Synonym Full Names

CCCTC-binding factor (zinc finger protein)-like

NCBI Official Symbol

Ctcfl

NCBI Official Synonym Symbols

Boris; OTTMUSG00000016680

UniProt Protein Name

Transcriptional repressor CTCFL

UniProt Synonym Protein Names

Brother of the regulator of imprinted sites; CCCTC-binding factor; CTCF paralog; CTCF-like protein

Protein Family

Transcriptional repressor

UniProt Gene Name

Ctcfl

UniProt Synonym Gene Names

Boris

UniProt Entry Name

CTCFL_MOUSE

NCBI Protein Information

transcriptional repressor CTCFL

UniProt Comments for Ctcfl

BORIS: Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters. Interacts with histones, PRMT7 and SETD1A. Interacts (via N-terminus) with BAG6/BAT3. Testis specific. Specifically expressed in primary spermatocytes. Belongs to the CTCF zinc-finger protein family.

Protein type: Cancer Testis Antigen (CTA); C2H2-type zinc finger protein; DNA-binding

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; histone binding; metal ion binding; nucleic acid binding; protein binding; sequence-specific DNA binding

Biological Process: chromatin modification; DNA methylation during gametogenesis; genetic imprinting; histone methylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of histone H3-K4 methylation; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter; transcription, DNA-dependent

Research Articles on Ctcfl

1. The TGFB pathway as most affected by embryonic Ctcfl expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Ctcfl recombinant protein

Reference Product	PubMed Publications
Myc recombinant protein	>2 publications with Ctcfl and Myc
Magea1 recombinant protein	>1 publications with Ctcfl and Magea1
Bag1 recombinant protein	>1 publications with Ctcfl and Bag1
Prss50 recombinant protein	>1 publications with Ctcfl and Prss50
Prmt7 recombinant protein	>1 publications with Ctcfl and Prmt7

Diseases associated with Ctcfl recombinant protein

Disease Name	Pubmed Publications
Carcinoma Antibodies	>18 publications with Ctcfl and Carcinoma
Breast Neoplasms Antibodies	>11 publications with Ctcfl and Breast Neoplasms
Skin Diseases Antibodies	>10 publications with Ctcfl and Skin Diseases
Ovarian Neoplasms Antibodies	>10 publications with Ctcfl and Ovarian Neoplasms
Lung Neoplasms Antibodies	>8 publications with Ctcfl and Lung Neoplasms
Ovarian Diseases Antibodies	>8 publications with Ctcfl and Ovarian Diseases
Lung Diseases Antibodies	>7 publications with Ctcfl and Lung Diseases
Neoplasms, Experimental Antibodies	>6 publications with Ctcfl and Neoplasms, Experimental
Genital Diseases, Male Antibodies	>6 publications with Ctcfl and Genital Diseases, Male
Infertility, Male Antibodies	>2 publications with Ctcfl and Infertility, Male

Organs/Tissues associated with Ctcfl recombinant protein

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>2 publications with Ctcfl and Skin