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Mesencephalic Astrocyte-Derived Neurotrophic Factor Protein (MANF) Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS969407

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in… More details

$245 / 0.005 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Mesencephalic astrocyte-derived neurotrophic factor protein (MANF), Active Protein
Full Product Name
Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor protein (MANF) (Active)
Product Gene Name
Product Synonym Gene Name
ARMET; ARP
Product Synonym Names
Arginine-rich protein
Sequence
LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
Host
E Coli
Form/Format
Lyophilized
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Generally, the shelf life of liquid form is 6 months at -20/-80 degree C. The shelf life of lyophilized form is 12 months at -20/-80 degree C.
Molecular Weight
18.2 kDa
Sequence Positions
25-182aa
OMIM
M83751 mRNA
3D Structure
ModBase 3D Structure for P55145
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Homo sapiens (Human)
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 ug/ml, corresponding to a specific activity of > 50 IU/mg.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Protein Note
Protein will be provided with aseptic processing and endotoxin removal.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MANF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MANF purchase

MBS969407 is an active protein targeting MANF. Also known as Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor protein (MANF) (Active). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MANF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mesencephalic astrocyte-derived neurotrophic factor
NCBI Official Synonym Full Names
mesencephalic astrocyte derived neurotrophic factor
NCBI Official Symbol
MANF
NCBI Official Synonym Symbols
ARP; ARMET
UniProt Protein Name
Mesencephalic astrocyte-derived neurotrophic factor
UniProt Synonym Protein Names
Arginine-rich protein; Protein ARMET
Protein Family
UniProt Gene Name
MANF
UniProt Synonym Gene Names
ARMET; ARP
NCBI Protein Information
mesencephalic astrocyte-derived neurotrophic factor

NCBI Summary for MANF

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]
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UniProt Comments for MANF

ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.2

Cellular Component: cytosol; endoplasmic reticulum; extracellular region; extracellular space; nucleus

Molecular Function: RNA binding

Biological Process: neurite development; platelet degranulation

Disease: Pancreatic Cancer
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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