ULBP1 Antibody – Rabbit Polyclonal

Figure 1. Western blot analysis of ULBP1 using anti-ULBP1 antibody (MBS1753654).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates
Lane 2: mouse brain tissue lysates
Lane 3: mouse heart tissue lysates
Lane 4: mouse lung tissue lysates
Lane 5: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ULBP1 antigen affinity purified polyclonal antibody (Catalog # MBS1753654) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460 ) with Tanon 5200 system. A specific band was detected for ULBP1 at approximately 28KD. The expected band size for ULBP1 is at 28KD.

Figure 2. IHC analysis of ULBP1 using anti ULBP1 antibody (MBS1753654).
ULBP1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ULBP1 Antibody (MBS1753654) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451 ) with DAB as the chromogen.

Figure 3. IHC analysis of ULBP1 using anti ULBP1 antibody (MBS1753654).
ULBP1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ULBP1 Antibody (MBS1753654) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen.

Figure 4. Flow Cytometry analysis of PC-3 cells using anti-ULBP1 antibody (MBS1753654).
Overlay histogram showing PC-3 cells stained with MBS1753654 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ULBP1 Antibody (MBS1753654,1μg/1x10⁶ cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.