Alpha-2-Macroglobulin (A2M) ELISA Kit for Human

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to alpha2 MG has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated alpha2 MG and incubated. A competitive inhibition reaction is launched between alpha2 MG (Standards or samples) and HRP-conjugated alpha2 MG with the pre-coated antibody specific for alpha2 MG. The more amount of alpha2 MG in samples, the less antibody bound by HRP-conjugated alpha2 MG. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of alpha2 MG in the sample. The color development is stopped and the intensity of the color is measured.

Small volumes of A2M elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS702999 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-2-macroglobulin (A2M) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing A2M. The ELISA analytical biochemical technique of the MBS702999 kit is based on A2M antibody-A2M antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect A2M antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, A2M. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).