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Abl Interactor 1 (ABI1) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280532

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ABI1 in samples. An antibody specific for ABI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABI1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Abl interactor 1 (ABI1), ELISA Kit
Full Product Name
Rat Abl interactor 1 (ABI1) ELISA Kit
Product Gene Name
Species Reactivity
Rat
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat ABI1. No significant cross-reactivity or interference between Rat ABI1 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
51,705 Da
3D Structure
ModBase 3D Structure for Q9QZM5
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ABI1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ABI1 purchase

MBS280532 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Abl interactor 1 (ABI1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ABI1. The ELISA analytical biochemical technique of the MBS280532 kit is based on ABI1 antibody-ABI1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ABI1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ABI1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ABI1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
abl interactor 1 isoform X2
NCBI Official Synonym Full Names
abl-interactor 1
NCBI Official Symbol
Abi1
NCBI Official Synonym Symbols
E3b1
UniProt Protein Name
Abl interactor 1
UniProt Synonym Protein Names
Abelson interactor 1; Abi-1; Eps8 SH3 domain-binding protein; Eps8-binding protein; e3B1
Protein Family
UniProt Gene Name
Abi1
UniProt Synonym Gene Names
Abi-1; Eps8-binding protein
NCBI Protein Information
abl interactor 1

UniProt Comments for ABI1

Abi-1: an SH3-containing adapter protein that regulates c-Abl-mediated phosphorylation of Mena. Binds the SH3 domains of epidermal growth factor receptor kinase substrate (EPS8) and of spectrin. May play a role in fusion of macropinocytic vesicles. Associated with congenital acute monocytic leukemia.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12.3

Cellular Component: cell junction; cell leading edge; cytoplasm; cytoskeleton; filopodium; growth cone; intracellular; lamellipodium; neuron projection; nucleus; postsynaptic density; postsynaptic membrane

Molecular Function: cadherin binding; protein binding; protein complex binding; protein tyrosine kinase activator activity; SH3 domain binding; transcription factor binding

Biological Process: actin polymerization or depolymerization; dendrite morphogenesis; peptidyl-tyrosine phosphorylation; somitogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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