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A53T Mutant Alpha Synuclein (A53T) Human Recombinant Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8005531

Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
A53T Mutant Alpha Synuclein, Active Protein
Full Product Name
Active Human Recombinant A53T Mutant Alpha Synuclein Protein Monomer
Product Gene Name
A53T active protein
Product Synonym Names
Alpha Synuclein Protein; A53T mutant alpha synuclein; A53T mutated SNCA; A53T Alpha synuclein monomer; Ala53thr mutant alpha synuclein; Active Alpha synuclein protein; Alpha-synuclein protein; Non-A beta component of AD amyloid protein; Non-A4 component of amyloid precursor protein; NACP protein; SNCA protein; NACP protein; PARK1 protein; Alpha synuclein monomers; SYN protein; Parkinson disease familial 1 Protein
Sequence
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Host
E Coli
Form/Format
PBS pH 7.4
Purity/Purification
>95%
Ion-Exchange Purified
Specificity
~14.46 kDa
Preparation and Storage
Store at-80 degree C.
3D Structure
ModBase 3D Structure for P37840 
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Human
Biological Activity
100 uM A53T alpha synuclein protein monomer seeded with 10 nM A53T alpha synuclein protein PFF in 25 uM Thioflavin T (PBS pH 7.4, 100 ul reaction volume) generated a fluorescence intensity of 28 000 Relative Fluorescence Units after incubation at 37 degree C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
Cellular Localization
Cytoplasm; Membrane; Nucleus
Tag
No Tag
Certificate of Analysis
Certified >95% pure using SDS-PAGE analysis.

Product Categories/Family for A53T active protein

Neuroscience; Neurodegeneration; Alzheimer's Disease; Tangles & Tau; Neuroscience; Neurodegeneration; Parkinson's Disease; Synuclein
Applications Tested/Suitable for A53T active protein
Western Blot (WB), SDS-PAGE, In Vitro Assay

SDS-Page of A53T active protein

SDS-PAGE of ~14 kDa A53T Alpha Synuclein Monomer (SPR-325)
Human Recombinant A53T Active Protein – Cat# MBS8005531

Testing Data of A53T active protein

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers (SPR-325). A much greater increase in fluorescence is seen when 100 uM monomer (SPR-325) is combined with 10 nM of fibrils (SPR-326) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.
Human Recombinant A53T Active Protein, Testing Data – Cat# MBS8005531

Product References and Citations for A53T active protein

1. "Genetics Home Reference: SNCA". US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757. 7. Polymeropoulos, M. H. (1998). Science. 276(5321), 2045-2047. 8. Conway, K.E., et al. (1998). Nat Med. 4(11):1318-20.

Other Notes

Small volumes of A53T active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for A53T purchase

MBS8005531 is an active protein targeting A53T. Also known as Active Human Recombinant A53T Mutant Alpha Synuclein Protein Monomer. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for A53T. It may not necessarily be applicable to this product.

NCBI GI #
4507109
NCBI GeneID
6622
NCBI Accession #
NP_000336.1
UniProt Primary Accession #
P37840 
UniProt Related Accession #
P37840
GenBank Nucleotide #
NP_000336.1
NCBI Official Full Name
alpha-synuclein isoform NACP140
NCBI Official Synonym Full Names
synuclein alpha
NCBI Official Symbol
SNCA
NCBI Official Synonym Symbols
PD1; NACP; PARK1; PARK4
NCBI Protein Information
alpha-synuclein

NCBI Summary for A53T

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]
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Research Articles on A53T

1. SNCA-Tri neurons displayed exacerbated nuclear aging, showing advanced aging signatures already at the Juvenile stage. Noteworthy, the Aged SNCA-Tri neurons showed more alpha-synuclein aggregates per cell versus the Juvenile.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with A53T active protein

 Reference Product  PubMed Publications
 SNCB active protein  >74 publications with A53T and SNCB
 MAPT active protein  >64 publications with A53T and MAPT
 SNCAIP active protein  >31 publications with A53T and SNCAIP
 PARK2 active protein  >30 publications with A53T and PARK2
 APP active protein  >16 publications with A53T and APP
 SLC6A3 active protein  >12 publications with A53T and SLC6A3
 MAPK1 active protein  >5 publications with A53T and MAPK1
 TOR1A active protein  >4 publications with A53T and TOR1A
 BAD active protein  >3 publications with A53T and BAD
 SQSTM1 active protein  >3 publications with A53T and SQSTM1

Pathways associated with A53T active protein

 Products by Pathway  Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Parkin-Ubiquitin Proteasomal System Pathway antibodies  Parkin-Ubiquitin Proteasomal System Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
 Parkinsons Disease Pathway antibodies  Parkinsons Disease Pathway Diagram

Diseases associated with A53T active protein

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1049 publications with A53T and Nervous System Diseases
 Brain Diseases Antibodies  >1028 publications with A53T and Brain Diseases
 Neurodegenerative Diseases Antibodies  >1016 publications with A53T and Neurodegenerative Diseases
 Movement Disorders Antibodies  >896 publications with A53T and Movement Disorders
 Parkinsonian Disorders Antibodies  >829 publications with A53T and Parkinsonian Disorders
 Parkinson Disease Antibodies  >755 publications with A53T and Parkinson Disease
 Lewy Body Disease Antibodies  >270 publications with A53T and Lewy Body Disease
 Nerve Degeneration Antibodies  >160 publications with A53T and Nerve Degeneration
 Disease Models, Animal Antibodies  >127 publications with A53T and Disease Models, Animal
 Drug Toxicity Antibodies  >71 publications with A53T and Drug Toxicity

Organs/Tissues associated with A53T active protein

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >986 publications with A53T and Nerve
 Brain Antibodies  >718 publications with A53T and Brain
 Blood Antibodies  >53 publications with A53T and Blood
 Embryonic Tissue Antibodies  >28 publications with A53T and Embryonic Tissue
 Kidney Antibodies  >19 publications with A53T and Kidney
 Heart Antibodies  >9 publications with A53T and Heart
 Eye Antibodies  >8 publications with A53T and Eye
 Skin Antibodies  >6 publications with A53T and Skin
 Bone Antibodies  >6 publications with A53T and Bone
 Liver Antibodies  >6 publications with A53T and Liver
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