Human aFGF Active Protein – Cat# MBS9718661

Fig.SDS-PAGE analysis of Human aFGF protein.

aFGF Human Active Protein

Name: aFGF Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS9718661
Price: 205 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9718661

aFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1-4), which results in receptor dimerization and… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

aFGF, Active Protein

Full Product Name

Human aFGF Protein

Product Gene Name

aFGF active protein

Product Synonym Names

Recombinant Human aFGF Protein (Fibroblast growth factor 1) expressed in E. coli; AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGFA; GLIO703; HBGF-1; HBGF1; Fibroblast growth factor 1; FGF1

Sequence

Amino acid sequence derived from mature form of human FGF acidic (AAA79245. 1) (Phe 16-Asp 155) was expressed, with an additional Met at the N-terminus .

Host

E Coli

Form/Format

Lyophilized from sterile PBS, pH 7.4

Purity/Purification

>95% as determined by SDS-PAGE

Preparation and Storage

Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Molecular Weight

16 kDa

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Species

Human

Protein Length

The recombinant human FGF acidic consists of 141 amino acids and has a calculated molecular mass of 16 kDa as estimated by SDS-PAGE under reducing conditions.

Activity

Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 50-200 pg/ml.

Usage Notes

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human aFGF protein in PBS, PH7.4 not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Other Notes

Small volumes of aFGF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for aFGF purchase

MBS9718661 is an active protein targeting aFGF. Also known as Human aFGF Protein. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for aFGF. It may not necessarily be applicable to this product.

NCBI GI #

181942

NCBI GeneID

2246

NCBI Accession #

AAA79245.1

UniProt Related Accession #

P05230

NCBI Official Full Name

beta-endothelial cell growth factor

NCBI Official Synonym Full Names

fibroblast growth factor 1

NCBI Official Symbol

FGF1

NCBI Official Synonym Symbols

AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha

Protein Family

Acidic fibroblast growth factor intracellular-binding protein

NCBI Protein Information

fibroblast growth factor 1

NCBI Summary for aFGF

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

Research Articles on aFGF

1. Gas5 regulated proliferation and apoptosis in growth plate by controlling FGF1 expression via miR-21 regulation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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