BACE1 Active Protein

Name: BACE1 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS8119979
Price: 415 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8119979

Background: Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla… More details

$415 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BACE1, Active Protein

Full Product Name

BACE1 Protein, Human, Recombinant

Product Gene Name

BACE1 active protein

Product Synonym Names

Human BACE1/ASP2 Protein; ASP2 Protein, Human; BACE Protein, Human; HSPC104 Protein, Human; beta-site APP-cleaving enzyme 1

Sequence

Met1-Thr45 7

Host

HEK293 Cells

Form/Format

Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Purity/Purification

>95% as determined by SDS-PAGE

Preparation and Storage

Samples are stable for up to twelve months from date of receipt at -70 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.

Molecular Weight

42,107 Da

OMIM

604252

3D Structure

ModBase 3D Structure for P56817

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Source

Human

Endotoxin

<1.0EU per ug of the protein as determined by the LAL method

Predicted N Terminal

Thr 22

Tag

C-cleavage

Protein Construction

The mature form of human BACE1 (NP_036236.1) extracellular domain (Met1-Thr 457) with a quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Bio Activity

Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2. Cleavage of ES004 can be measured using excitation and emission wavelength of 320 and 405nM, respectively. The specific activity is >3.5pmoles/min/ug.

Product References and Citations for BACE1 active protein

Christensen MA, et al. (2004) Transcriptional regulation of BACE1, the beta-amyloid precursor protein beta-secretase, by Sp1. Mol Cell Biol. 24(2):865-74.Stockley JH, et al. (2007) The proteins BACE1 and BACE2 and beta-secretase activity in normal and Alzheimer's disease brain. Biochem Soc Trans. 35(Pt 3): 574-6.Savonenko AV, et al. (2008) Alteration of BACE1-dependent NRG1/ErbB4 signaling and schizophrenia-like phenotypes in BACE1-null mice. Proc Natl Acad Sci U S A. 105(14): 5585-90.Hayley M, et al. (2009) Calcium enhances the proteolytic activity of BACE1: An in vitro biophysical and biochemical characterization of the BACE1-calcium interaction. Biochim Biophys Acta. 1788(9): 1933-8.

Other Notes

Small volumes of BACE1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BACE1 purchase

MBS8119979 is an active protein targeting BACE1. Also known as BACE1 Protein, Human, Recombinant. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for BACE1. It may not necessarily be applicable to this product.

NCBI GI #

6912266

NCBI GeneID

23621

NCBI Accession #

NP_036236.1

UniProt Primary Accession #

P56817

UniProt Secondary Accession #

Q9BYB9; Q9BYC0; Q9BYC1; Q9UJT5; Q9ULS1; A0M8W7; B0YIU9; E9PE65; H7BXJ9

UniProt Related Accession #

P56817

GenBank Nucleotide #

NM_012104.4

NCBI Official Full Name

beta-secretase 1 isoform A preproprotein

NCBI Official Synonym Full Names

beta-site APP-cleaving enzyme 1

NCBI Official Symbol

BACE1

NCBI Official Synonym Symbols

ASP2; BACE; HSPC104

UniProt Protein Name

Beta-secretase 1

UniProt Synonym Protein Names

Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2

Protein Family

Beta-secretase

UniProt Gene Name

BACE1

UniProt Synonym Gene Names

BACE; KIAA1149; ASP2; Asp 2

UniProt Entry Name

BACE1_HUMAN

NCBI Protein Information

beta-secretase 1; asp 2; memapsin-2; APP beta-secretase; aspartyl protease 2; beta-site APP cleaving enzyme 1; beta-secretase 1 precursor variant 1; transmembrane aspartic proteinase Asp2; membrane-associated aspartic protease 2; beta-site amyloid beta A4

UniProt Comments for BACE1

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.

Protein type: Protease; EC 3.4.23.46; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.2-q23.3

Cellular Component: multivesicular body; Golgi apparatus; cell surface; cytoplasmic vesicle membrane; integral to plasma membrane; axon; endoplasmic reticulum; late endosome; integral to membrane; plasma membrane; trans-Golgi network; endosome

Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity

Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with BACE1 active protein

Reference Product	PubMed Publications
APP active protein	>1146 publications with BACE1 and APP
PSEN1 active protein	>287 publications with BACE1 and PSEN1
RTN3 active protein	>19 publications with BACE1 and RTN3
GGA3 active protein	>13 publications with BACE1 and GGA3
GGA1 active protein	>13 publications with BACE1 and GGA1
NCSTN active protein	>5 publications with BACE1 and NCSTN
GGA2 active protein	>3 publications with BACE1 and GGA2
PLSCR1 active protein	>1 publications with BACE1 and PLSCR1

Pathways associated with BACE1 active protein

Products by Pathway	Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimers Disease Pathway antibodies	Alzheimers Disease Pathway Diagram

Diseases associated with BACE1 active protein

Disease Name	Pubmed Publications
Alzheimer Disease Antibodies	>1295 publications with BACE1 and Alzheimer Disease
Brain Diseases Antibodies	>1218 publications with BACE1 and Brain Diseases
Necrosis Antibodies	>97 publications with BACE1 and Necrosis
Memory Disorders Antibodies	>73 publications with BACE1 and Memory Disorders
Neurobehavioral Manifestations Antibodies	>64 publications with BACE1 and Neurobehavioral Manifestations
Inflammation Antibodies	>60 publications with BACE1 and Inflammation
Cognition Disorders Antibodies	>58 publications with BACE1 and Cognition Disorders
Learning Disorders Antibodies	>56 publications with BACE1 and Learning Disorders
Nerve Degeneration Antibodies	>24 publications with BACE1 and Nerve Degeneration
Brain Injuries Antibodies	>10 publications with BACE1 and Brain Injuries

Organs/Tissues associated with BACE1 active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>1046 publications with BACE1 and Brain
Blood Antibodies	>176 publications with BACE1 and Blood
Kidney Antibodies	>87 publications with BACE1 and Kidney
Vascular Antibodies	>64 publications with BACE1 and Vascular
Liver Antibodies	>27 publications with BACE1 and Liver
Muscle Antibodies	>26 publications with BACE1 and Muscle
Embryonic Tissue Antibodies	>26 publications with BACE1 and Embryonic Tissue
Ovary Antibodies	>23 publications with BACE1 and Ovary
Ear Antibodies	>17 publications with BACE1 and Ear
Skin Antibodies	>13 publications with BACE1 and Skin