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BLMH Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201145

BLMH, also known as bleomycin hydrolase, is a member of the papain superfamily of the cysteine protease and the peptidase C1 family. It is a cytoplasmic cysteinepeptidase commonly found as a homohexamer. The normal physiological role of BLMH is unknown, but… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BLMH, Active Protein
Full Product Name
Recombinant BLMH
Product Gene Name
Product Synonym Names
Bmh; Bh; Blm hydrolase; bleomycin hydrolase; AI035728; Bleomycin hydrolase; Blm hydrolase; Blmh; EC 3.4.22.40
Sequence
MGSSHHHHHH SSGLVPRGSH MGS MNNAGLN SEKVSALIQK LNSDPQFVLA QNVGTTHDLL DICLRRATVQ GAQHVFQHVV PQEGKPVTNQ KSSGRCWIFS CLNVMRLPFM KKFNIEEFEF SQSYLFFWDK VERCYFFLNA FVDTAQKKEP EDGRLVQYLL MNPTNDGGQW DMLVNIVEKY GVVPKKCFPE SHTTEATRRM NDILNHKMRE FCIRLRNLVH SGATKGEISS TQDAMMEEIF RVVCICLGNP PETFTWEYRD KDKNYHKIGP ITPLQFYKEH VKPLFNMEDK ICFVNDPRPQ HKYNKLYTVD YLSNMVGGRK TLYNNQPIDF LKKMVAASIK DGEAVWFGCD VGKHFNGKLG LSDMNVYDHE LVFGVSLKNM NKAERLAFGE SLMTHAMTFT AVSEKDNQEG TFVKWRVENS WGEDHGHKGY LCMTDEWFSE YVYEVVVDKK HVPEEVLAVL EQEPIVLPAW DPMGALAE
Host
E Coli
Form/Format
Liquid; In 20mM Tris-HCl (pH8.0) Containing 1mM DTT, 0.1M NaCl, 30% Glycerol.
Purity/Purification
>90% by SDS-PAGE
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
Molecular Weight
54.9 kDa (478aa), confirmed by MALDI-TOF
Sequence Positions
1-455aa
3D Structure
ModBase 3D Structure for Q8R016
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Mouse
Tag
His-Tag
Biological Activity
Specific activity is > 1,500 pmole/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Met-AMC to Methionine and AMC per minute at pH7.5 at 37C.

Product Categories/Family for BLMH active protein

Applications Tested/Suitable for BLMH active protein
SDS-PAGE, Enzyme Activity

SDS-Page of BLMH active protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
BLMH Active Protein – Cat# MBS201145

Other Notes

Small volumes of BLMH active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BLMH purchase

MBS201145 is an active protein targeting BLMH. Also known as Recombinant BLMH. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for BLMH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
bleomycin hydrolase
NCBI Official Synonym Full Names
bleomycin hydrolase
NCBI Official Symbol
Blmh
NCBI Official Synonym Symbols
Bh; Bmh; AI035728
UniProt Protein Name
Bleomycin hydrolase
Protein Family
UniProt Gene Name
Blmh
UniProt Synonym Gene Names
BH; BLM hydrolase; BMH
NCBI Protein Information
bleomycin hydrolase

NCBI Summary for BLMH

The encoded protein is a cytoplasmic cysteine peptidase involved in inactivation of bleomycin, a glycopeptide which is a component of combination chemotherapy regimens for cancer. This encoded enzyme is highly conserved, and it contains the signature active site residues of cysteine protease papain superfamily enzymes. It is postulated that this enzyme has protective effects against bleomycin-induced pulmonary fibrosis and bleomycin tumor resistance. [provided by RefSeq, Jan 2010]
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UniProt Comments for BLMH

BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.40; Protease

Chromosomal Location of Human Ortholog: 11|11 B5

Cellular Component: cytoplasm; nucleus

Molecular Function: aminopeptidase activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; hydrolase activity; identical protein binding; peptidase activity

Biological Process: homocysteine catabolic process; proteolysis; response to drug; response to toxin
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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