CD33/SIGLEC-3 (CD33) Active Protein

Name: CD33 Active Protein; CD33/SIGLEC-3; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844963
Price: 335 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844963

Background: Myeloid cell surface antigen CD33 also known as SIGLEC3, Siglecs (sialic acid binding Ig-like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CD33/SIGLEC-3, Active Protein

Full Product Name

Human CellExp CD33 / SIGLEC-3, human recombinant

Product Gene Name

CD33 active protein

Product Synonym Names

CD33; SIGLEC3; gp67

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with C-terminal 6×His tag, has a calculated MW of 27.6 kDa expressed. The predicted N-terminus is Asp 18. Protein migrates as 40-45 kDa in reduced SDS-PAGE.

OMIM

159590

3D Structure

ModBase 3D Structure for P20138

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is typically 0.2-1.2 mug/ml.

Results

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is typically 0.2-1.2 mug/ml.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for CD33 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Proteins and E

Applications Tested/Suitable for CD33 active protein

SDS-PAGE

Other Notes

Small volumes of CD33 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD33 purchase

MBS844963 is an active protein targeting CD33. Also known as Human CellExp CD33 / SIGLEC-3, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CD33. It may not necessarily be applicable to this product.

NCBI GI #

130980072

NCBI GeneID

945

NCBI Accession #

NP_001076087.1

UniProt Primary Accession #

P20138

UniProt Secondary Accession #

Q8TD24; B4E3P8; C9JEN7; F8WAL2

UniProt Related Accession #

P20138

GenBank Nucleotide #

NM_001082618.1

NCBI Official Full Name

myeloid cell surface antigen CD33 isoform 2

NCBI Official Synonym Full Names

CD33 molecule

NCBI Official Symbol

CD33

NCBI Official Synonym Symbols

p67; SIGLEC3; SIGLEC-3

UniProt Protein Name

Myeloid cell surface antigen CD33

UniProt Synonym Protein Names

Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD_antigen: CD33

Protein Family

Myeloid cell surface antigen

UniProt Gene Name

CD33

UniProt Synonym Gene Names

SIGLEC3; Siglec-3

UniProt Entry Name

CD33_HUMAN

NCBI Protein Information

myeloid cell surface antigen CD33

UniProt Comments for CD33

CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity; carbohydrate binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion

Research Articles on CD33

1. 87.8% of AMLs express CD33. Additionally, 9.4% of AMLs express CD123 without concomitant CD33 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.