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Complement Component 5a (C5A) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2162242
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MBS2162242 is an active protein targeting C5a. Also known as Active Complement Component 5a (C5a). Host: 293F cell. More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Complement Component 5a (C5a), Active Protein
Full Product Name
Active Complement Component 5a (C5a)
Product Gene Name
C5a active protein
Product Synonym Names
Complement Component 5a; C5a active protein (eukaryotic,293F cell), Mouse4
Sequence
Asn679~Arg 755
Host
293F cell
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Purity/Purification
Greater than 90% by SDS-PAGE
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
3D Structure
ModBase 3D Structure for P06684
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Mouse
Expression System
Eukaryotic expression
Tag
N-terminal His Tag and C-terminal Fc Region of Human IgG1
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
9.4
Research Area
Infection immunity, Immune molecule, Autoimmunity

Product Categories/Family for C5a active protein

Active proteins
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for C5a active protein
Cell culture, Activity Assays

SDS-PAGE of C5a active protein

Figure. SDS-PAGE
C5A Active Protein – Cat# MBS2162242

Activity of C5a active protein

Complement Component 5a (C5a) is a component of the complement system which plays a key role in promoting migration and adherence of neutrophils and monocytes to vessel walls. The activity of recombinant human C5a was also measured by its ability to induce N-acetyl-beta-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. 3.2*106 differentiated U937 cells were added to the 24-well plate and different concentrations of rhC5a was added to the 24-well plate and incubated at 37 degree C for 3min. The cells were centrifuged at 400 g for 3min, and the supernatant contained N-acetyl-beta-D-glucosaminidase. The enzyme activity of N-acetyl-beta-D-glucosaminidase was measured by the substrate of 4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside. The result was shown in figure 1, It was obvious that rhC5a can significantly induce N-acetyl-beta-D-glucosaminidase release.
C5A Active Protein, Activity – Cat# MBS2162242

Other Notes

Small volumes of C5a active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for C5a. It may not necessarily be applicable to this product.

NCBI GI #
6754164
NCBI GeneID
15139
NCBI Accession #
NP_034536.1
UniProt Primary Accession #
P06684
UniProt Secondary Accession #
A2AS36
UniProt Related Accession #
P06684
GenBank Nucleotide #
NM_010406.2
NCBI Official Full Name
complement C5 preproprotein
NCBI Official Synonym Full Names
hemolytic complement
NCBI Official Symbol
Hc
NCBI Official Synonym Symbols
C5; He; C5a
UniProt Protein Name
Complement C5
UniProt Synonym Protein Names
Hemolytic complementCleaved into the following 4 chains:Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain
UniProt Gene Name
C5
UniProt Synonym Gene Names
Hc
UniProt Entry Name
CO5_MOUSE
NCBI Protein Information
complement C5

UniProt Comments for C5a

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted, signal peptide; Secreted

Cellular Component: membrane attack complex; extracellular space; extracellular region

Molecular Function: endopeptidase inhibitor activity

Biological Process: positive regulation of angiogenesis; in utero embryonic development; immune system process; cytolysis; complement activation, alternative pathway; innate immune response; inflammatory response; complement activation, classical pathway
Show more

Research Articles on C5a

1. Protein kinase C-delta mediates sepsis-induced activation of complement 5a and urokinase-type plasminogen activator signaling in macrophages

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with C5a active protein

 Products by Pathway  Pathway Diagram
 Activation Of C3 And C5 Pathway antibodies  Activation Of C3 And C5 Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram

Diseases associated with C5a active protein

 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >3136 publications with C5a and Heart Diseases
 Nervous System Diseases Antibodies  >2954 publications with C5a and Nervous System Diseases
 Inflammation Antibodies  >1038 publications with C5a and Inflammation
 Lung Diseases Antibodies  >826 publications with C5a and Lung Diseases
 Liver Diseases Antibodies  >806 publications with C5a and Liver Diseases
 Pain Antibodies  >696 publications with C5a and Pain
 Kidney Diseases Antibodies  >650 publications with C5a and Kidney Diseases
 Necrosis Antibodies  >498 publications with C5a and Necrosis
 Hematologic Diseases Antibodies  >450 publications with C5a and Hematologic Diseases
 Insulin Resistance Antibodies  >449 publications with C5a and Insulin Resistance

Organs/Tissues associated with C5a active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2616 publications with C5a and Brain
 Liver Antibodies  >1362 publications with C5a and Liver
 Lung Antibodies  >1322 publications with C5a and Lung
 Intestine Antibodies  >341 publications with C5a and Intestine
 Embryonic Tissue Antibodies  >167 publications with C5a and Embryonic Tissue
 Mammary Gland Antibodies  >47 publications with C5a and Mammary Gland
 Pituitary Gland Antibodies  >42 publications with C5a and Pituitary Gland
 Uncharacterized Tissue Antibodies  >1 publications with C5a and Uncharacterized Tissue
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